BMRB Entry 25406

Title:
Titin M10 H56P mutation
Deposition date:
2014-12-29
Original release date:
2015-08-25
Authors:
Wright, Nathan; Rudloff, Michael
Citation:

Citation: Rudloff, Michael; Woosley, Alec; Wright, Nathan. "Biophysical characterization of naturally occurring titin M10 mutations"  Protein Sci. 24, 946-955 (2015).
PubMed: 25739468

Assembly members:

Assembly members:
H56P, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H56P1

Entities:

Entity 1, H56P 107 residues - Formula weight is not available

1   HISHISHISHISHISHISGLYSERARGGLY
2   ILEPROPROLYSILEGLUALALEUPROSER
3   ASPILESERILEASPGLUGLYLYSVALLEU
4   THRVALALACYSALAPHETHRGLYGLUPRO
5   THRPROGLUVALTHRTRPSERCYSGLYGLY
6   ARGLYSILEHISSERGLNGLUGLNGLYARG
7   PHEPROILEGLUASNTHRASPASPLEUTHR
8   THRLEUILEILEMETASPVALGLNLYSGLN
9   ASPGLYGLYLEUTYRTHRLEUSERLEUGLY
10   ASNGLUPHEGLYSERASPSERALATHRVAL
11   ASNILEHISILEARGSERILE

Samples:

sample_1: H56P, [U-99% 15N], 1 mM; D2O, [U-2H], 10%; sodium phosphate 10 mM; sodim fluoride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 25303 25305
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks