BMRB Entry 25303

Name: Titin M10 bound to obscurin ig1
Published: 2015-08-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25303

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Titin M10 bound to obscurin ig1

Deposition date:

2014-10-29

Original release date:

2015-08-25

Authors:

Wright, Nathan; Rudloff, Michael; Woosley, Alec

Citation:

Citation: Rudloff, Michael; Woosley, Alec; Wright, Nathan. "Biophysical characterization of naturally occurring titin M10 mutations" Protein Sci. 24, 946-955 (2015).
PubMed: 25739468

Assembly members:

Assembly members:
titin_M10, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
titin_M10: GSRGIPPKIEALPSDISIDE GKVLTVACAFTGEPTPEVTW SCGGRKIHSQEQGRFHIENT DDLTTLIIMDVQKQDGGLYT LSLGNEFGSDSATVNIHIRS I

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	79
1H chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	titin M10	1

Entities:

Entity 1, titin M10 101 residues - Formula weight is not available

1

GLY

SER

ARG

GLY

ILE

PRO

LYS

ILE

GLU

2

ALA

LEU

PRO

SER

ASP

ILE

SER

ILE

ASP

GLU

3

GLY

LYS

VAL

LEU

THR

VAL

ALA

CYS

ALA

PHE

4

THR

GLY

GLU

PRO

THR

PRO

GLU

VAL

THR

TRP

5

SER

CYS

GLY

ARG

LYS

ILE

HIS

SER

GLN

6

GLU

GLN

GLY

ARG

PHE

HIS

ILE

GLU

ASN

THR

7

ASP

LEU

THR

LEU

ILE

MET

ASP

8

VAL

GLN

LYS

GLN

ASP

GLY

LEU

TYR

THR

9

LEU

SER

LEU

GLY

ASN

GLU

PHE

GLY

SER

ASP

10

SER

ALA

THR

VAL

ASN

ILE

HIS

ILE

ARG

SER

11

ILE

Samples:

sample_1: titin M10, [U-99% 13C; U-99% 15N], 0.5 – 2.5 mM; Tris 20 mM; NaCl 20 mM; NaN3 0.35 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: .02 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	25305 25406
PDB	2WP3 2WWK 2WWM 2Y9R 3KNB 3Q5O 4C4K 4UOW