BMRB Entry 25305

Title:
titin M10 domain
Deposition date:
2014-10-29
Original release date:
2015-08-25
Authors:
Wright, Nathan; Rudloss, Michael; Woosley, Alec
Citation:

Citation: Rudloff, Michael; Woosley, Alec; Wright, Nathan. "Biophysical characterization of naturally occurring titin M10 mutations"  Protein Sci. 24, 946-955 (2015).
PubMed: 25739468

Assembly members:

Assembly members:
titin_M10, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1titin M101

Entities:

Entity 1, titin M10 101 residues - Formula weight is not available

1   GLYSERARGGLYILEPROPROLYSILEGLU
2   ALALEUPROSERASPILESERILEASPGLU
3   GLYLYSVALLEUTHRVALALACYSALAPHE
4   THRGLYGLUPROTHRPROGLUVALTHRTRP
5   SERCYSGLYGLYARGLYSILEHISSERGLN
6   GLUGLNGLYARGPHEHISILEGLUASNTHR
7   ASPASPLEUTHRTHRLEUILEILEMETASP
8   VALGLNLYSGLNASPGLYGLYLEUTYRTHR
9   LEUSERLEUGLYASNGLUPHEGLYSERASP
10   SERALATHRVALASNILEHISILEARGSER
11   ILE

Samples:

sample_1: titin M10, [U-99% 13C; U-99% 15N], 0.5 – 2.5 mM; Tris 20 mM; NaCl 20 mM; NaN3 0.35 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: .02 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks