BMRB Entry 25094

Name: full-length EcMazE-DNA complex
Published: 2015-02-02

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25094

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

full-length EcMazE-DNA complex

Deposition date:

2014-07-15

Original release date:

2015-02-02

Authors:

Zangger, Klaus; van Nuland, Nico

Citation:

Citation: Zorzini, Valentina; Buts, Lieven; Schrank, Evelyne; Sterckx, Yann; Respondek, Michal; Engelberg-Kulka, Hanna; Loris, Remy; Zangger, Klaus; van Nuland, Nico. "Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding" Nucleic Acids Res. 43, 1241-1256 (2015).
PubMed: 25564525

Assembly members:

Assembly members:
full_EcMazE-DNA, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30-mazE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
full_EcMazE-DNA: MIHSSVKRWGNSPAVRIPAT LMQALNLNIDDEVKIDLVDG KLIIEPVRKEPVFTLAELVN DITPENLHENIDWGEPKDKE VW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	81
15N chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EcMazE, 1	1
2	EcMazE, 2	1

Entities:

Entity 1, EcMazE, 1 82 residues - Formula weight is not available

1

MET

ILE

HIS

SER

VAL

LYS

ARG

TRP

GLY

2

ASN

SER

PRO

ALA

VAL

ARG

ILE

PRO

ALA

THR

3

LEU

MET

GLN

ALA

LEU

ASN

LEU

ASN

ILE

ASP

4

ASP

GLU

VAL

LYS

ILE

ASP

LEU

VAL

ASP

GLY

5

LYS

LEU

ILE

GLU

PRO

VAL

ARG

LYS

GLU

6

PRO

VAL

PHE

THR

LEU

ALA

GLU

LEU

VAL

ASN

7

ASP

ILE

THR

PRO

GLU

ASN

LEU

HIS

GLU

ASN

8

ILE

ASP

TRP

GLY

GLU

PRO

LYS

ASP

LYS

GLU

9

VAL

TRP

Samples:

sample_1: full EcMazE-DNA, [U-99% 13C; U-99% 15N], 0.2 – 0.3 mM; DNA0.0 – 0.4 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 298 K; pH: 6.5; pressure: 1 atm; ionic strength: 50 mM

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	25086 25092 25093
PDB	1MVF 1UB4 2MRN 2MRU
DBJ	BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL	CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB	AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF	NP_311670 NP_417263 WP_000581935 WP_000581936 WP_000581937
SP	P0AE72 P0AE73
AlphaFold	P0AE72 P0AE73