BMRB Entry 25086

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25086
MolProbity Validation Chart

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Title:
truncated EcMazE
Deposition date:
2014-07-12
Original release date:
2015-02-02
Authors:
Zorzini, Valentina; Buts, Lieven; Loris, Remy; van Nuland, Nico
Citation:

Citation: Zorzini, Valentina; Buts, Lieven; Schrank, Evelyne; Sterckx, Yann; Respondek, Michal; Engelberg-Kulka, Hanna; Loris, Remy; Zangger, Klaus; van Nuland, Nico. "Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding"  Nucleic Acids Res. 43, 1241-1256 (2015).
PubMed: 25564525

Assembly members:

Assembly members:
truncEcMazE, polymer, 67 residues, 7675.774 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-mazE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
truncEcMazE: NHKVHHHHHHMSDDDDKGIH SSVKRWGNSPAVRIPATLMQ ALNLNIDDEVKIDLVDGKLI IEPVRKE

Data sets:
Data typeCount
1H chemical shifts442
13C chemical shifts269
15N chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1truncated EcMazE_11
2truncated EcMazE_21

Entities:

Entity 1, truncated EcMazE_1 67 residues - 7675.774 Da.

1   ASNHISLYSVALHISHISHISHISHISHIS
2   METSERASPASPASPASPLYSGLYILEHIS
3   SERSERVALLYSARGTRPGLYASNSERPRO
4   ALAVALARGILEPROALATHRLEUMETGLN
5   ALALEUASNLEUASNILEASPASPGLUVAL
6   LYSILEASPLEUVALASPGLYLYSLEUILE
7   ILEGLUPROVALARGLYSGLU

Samples:

sample_1: truncEcMazE, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 298 K; pH: 6.5; pressure: 1 atm; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian VNMRS 600 MHz
  • Varian VNMRS 800 MHz

Related Database Links:

BMRB 25092 25093 25094
PDB
DBJ BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF NP_311670 NP_417263 WP_000581935 WP_000581936 WP_000581937
SP P0AE72 P0AE73
AlphaFold P0AE72 P0AE73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks