BMRB Entry 25093

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25093

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Title:
full-length EcMazE
Deposition date:
2014-07-15
Original release date:
2015-02-02
Authors:
Zangger, Klaus; van Nuland, Nico
Citation:

Citation: Zorzini, Valentina; Buts, Lieven; Schrank, Evelyne; Sterckx, Yann; Respondek, Michal; Engelberg-Kulka, Hanna; Loris, Remy; Zangger, Klaus; van Nuland, Nico. "Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding"  Nucleic Acids Res. 43, 1241-1256 (2015).
PubMed: 25564525

Assembly members:

Assembly members:
full_EcMazE_free, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-mazE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
full_EcMazE_free: MIHSSVKRWGNSPAVRIPAT LMQALNLNIDDEVKIDLVDG KLIIEPVRKEPVFTLAELVN DITPENLHENIDWGEPKDKE VW

Data sets:
Data typeCount
1H chemical shifts511
13C chemical shifts323
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EcMazE, 11
2EcMazE, 21

Entities:

Entity 1, EcMazE, 1 82 residues - Formula weight is not available

1   METILEHISSERSERVALLYSARGTRPGLY
2   ASNSERPROALAVALARGILEPROALATHR
3   LEUMETGLNALALEUASNLEUASNILEASP
4   ASPGLUVALLYSILEASPLEUVALASPGLY
5   LYSLEUILEILEGLUPROVALARGLYSGLU
6   PROVALPHETHRLEUALAGLULEUVALASN
7   ASPILETHRPROGLUASNLEUHISGLUASN
8   ILEASPTRPGLYGLUPROLYSASPLYSGLU
9   VALTRP

Samples:

sample_1: full EcMazE free, [U-99% 13C; U-99% 15N], 1 mM; H20 90%; D2O 10%

sample_2: full EcMazE free, [U-99% 13C; U-99% 15N], 1 mM; D2O 100%

sample_conditions_1: temperature: 298 K; pH: 6.5; pressure: 1 atm; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 25086 25092 25094
PDB
DBJ BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF NP_311670 NP_417263 WP_000581935 WP_000581936 WP_000581937
SP P0AE72 P0AE73
AlphaFold P0AE72 P0AE73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks