BMRB Entry 2410
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR2410
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Citation: Stoll, Raphael; Renner, Christian; Muhlhahn, Peter; Hansen, Silke; Schumacher, Ralf; Hesse, Friederike; Kaluza, Brigitte; Engh, Richard; Voelter, Wolfgang; Holak, Tad. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C assignment of
the N-terminal domain of the human oncoprotein MDM2 that binds to p53" J. Biomol. NMR 17, 91-92 (2000).
Assembly members:
N-terminal domain of the human murine double minute clone 2 protein, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-terminal domain of the human murine double minute clone 2 protein: MCNTNMSVPTDGAVTTSQIP
ASEQETLVRPKPLLLKLLKS
VGAQKDTYTMKEVLFYLGQY
IMTKRLYDEKQQHIVYCSND
LLGDLFGVPSFSVKEHRKIY
TMIYRNLVVVNQQESSDSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 150 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 131 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-terminal domain of human MDM2 | 1 |
Entities:
Entity 1, N-terminal domain of human MDM2 119 residues - Formula weight is not available
| 1 | MET | CYS | ASN | THR | ASN | MET | SER | VAL | PRO | THR | ||||
| 2 | ASP | GLY | ALA | VAL | THR | THR | SER | GLN | ILE | PRO | ||||
| 3 | ALA | SER | GLU | GLN | GLU | THR | LEU | VAL | ARG | PRO | ||||
| 4 | LYS | PRO | LEU | LEU | LEU | LYS | LEU | LEU | LYS | SER | ||||
| 5 | VAL | GLY | ALA | GLN | LYS | ASP | THR | TYR | THR | MET | ||||
| 6 | LYS | GLU | VAL | LEU | PHE | TYR | LEU | GLY | GLN | TYR | ||||
| 7 | ILE | MET | THR | LYS | ARG | LEU | TYR | ASP | GLU | LYS | ||||
| 8 | GLN | GLN | HIS | ILE | VAL | TYR | CYS | SER | ASN | ASP | ||||
| 9 | LEU | LEU | GLY | ASP | LEU | PHE | GLY | VAL | PRO | SER | ||||
| 10 | PHE | SER | VAL | LYS | GLU | HIS | ARG | LYS | ILE | TYR | ||||
| 11 | THR | MET | ILE | TYR | ARG | ASN | LEU | VAL | VAL | VAL | ||||
| 12 | ASN | GLN | GLN | GLU | SER | SER | ASP | SER | SER |
Samples:
sample_1: N-terminal domain of the human murine double minute clone 2 protein, [U-15N], 0.1 – 0.5 mM
sample_2: N-terminal domain of the human murine double minute clone 2 protein, [U-15N; U-13C], 0.1 – 0.5 mM
Ex-cond_1: pH: 7.4; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DMX 750 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks