BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18876

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Complex of MDM2(3-109) and p73 TAD(10-25)   PubMed: 25591003

Deposition date: 2012-12-05 Original release date: 2015-06-01

Authors: Shin, Jae-Sun; Ha, Jihyang; Chi, Seung-Wook

Citation: Shin, Jae-Sun; Ha, Ji-Hyang; Lee, Dong-Hwa; Ryu, Kyoung-Seok; Bae, Kwang-Hee; Park, Byoung-Chul; Park, Sung-Goo; Yi, Gwan-Su; Chi, Seung-Wook. "Structural convergence of unstructured p53 family transactivation domains in MDM2 recognition"  Cell Cycle 14, 533-543 (2015).

Assembly members:
entity_1, polymer, 107 residues, 12275.353 Da.
entity_2, polymer, 16 residues, 1791.864 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):
entity_1: NTNMSVPTDGAVTTSQIPAS EQETLVRPKPLLLKLLKSVG AQKDTYTMKEVLFYLGQYIM TKRLYDEKQQHIVYCSNDLL GDLFGVPSFSVKEHRKIYTM IYRNLVV
entity_2: DGGTTFEHLWSSLEPD

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts111
1H chemical shifts762

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MDM2(3-109)1
2p73_TAD(10-25)2

Entities:

Entity 1, MDM2(3-109) 107 residues - 12275.353 Da.

1   ASNTHRASNMETSERVALPROTHRASPGLY
2   ALAVALTHRTHRSERGLNILEPROALASER
3   GLUGLNGLUTHRLEUVALARGPROLYSPRO
4   LEULEULEULYSLEULEULYSSERVALGLY
5   ALAGLNLYSASPTHRTYRTHRMETLYSGLU
6   VALLEUPHETYRLEUGLYGLNTYRILEMET
7   THRLYSARGLEUTYRASPGLULYSGLNGLN
8   HISILEVALTYRCYSSERASNASPLEULEU
9   GLYASPLEUPHEGLYVALPROSERPHESER
10   VALLYSGLUHISARGLYSILETYRTHRMET
11   ILETYRARGASNLEUVALVAL

Entity 2, p73_TAD(10-25) 16 residues - 1791.864 Da.

1   ASPGLYGLYTHRTHRPHEGLUHISLEUTRP
2   SERSERLEUGLUPROASP

Samples:

MDM2-p73TAD: MDM2(3-109), [U-95% 13C; U-95% 15N], 0.9 mM; p73 TAD(10-25), [U-95% 13C; U-95% 15N], 0.9 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCMDM2-p73TADisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticMDM2-p73TADisotropicsample_conditions_1
3D CBCA(CO)NHMDM2-p73TADisotropicsample_conditions_1
3D HNCOMDM2-p73TADisotropicsample_conditions_1
3D HNCACBMDM2-p73TADisotropicsample_conditions_1
3D HBHA(CO)NHMDM2-p73TADisotropicsample_conditions_1
3D HCCH-TOCSYMDM2-p73TADisotropicsample_conditions_1
3D HCCH-COSYMDM2-p73TADisotropicsample_conditions_1
3D 1H-15N NOESYMDM2-p73TADisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticMDM2-p73TADisotropicsample_conditions_1
3D 1H-13C NOESY aromaticMDM2-p73TADisotropicsample_conditions_1

Software:

X-PLOR NIH v2.26, Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Guntert, Mumenthaler and Wuthrich, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 11569 18755 2410
PDB
DBJ BAB11975 BAF83030 BAJ17752
EMBL CAA78055 CAD23251 CAD36959 CAD79457 CAH89564
GB AAA60568 AAF28866 AAG42840 AAI42265 AAI48523
PRF 1814460A
REF NP_001003103 NP_001092577 NP_001124685 NP_001138809 NP_001138811
SP P56951 Q00987

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts