BMRB Entry 20079

Name: NMR solution structure of the loopregion Tyr67 - Leu77 of visual arrestin in complex with lightactivated rhodopsin
Published: 2010-01-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20079

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR solution structure of the loopregion Tyr67 - Leu77 of visual arrestin in complex with lightactivated rhodopsin

Deposition date:

2009-03-29

Original release date:

2010-01-07

Authors:

Feuerstein, Sophie; Hartmann, Rudolf; Koenig, Bernd

Citation:

Citation: Feuerstein, Sophie; Pulvermuller, Alexander; Hartmann, Rudolf; Granzin, Joachim; Stoldt, Matthias; Henklein, Peter; Ernst, Oliver; Heck, Martin; Willbold, Dieter; Koenig, Bernd. "Helix formation in arrestin accompanies recognition of photoactivated rhodopsin." Biochemistry 48, 10733-10742 (2009).
PubMed: 19835414

Assembly members:

Assembly members:
Arr67_77, polymer, 11 residues, Formula weight is not available
rhodopsin, polymer, . residues, Formula weight is not available

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Arr67_77: YGQEDIDVMGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Arr67_77	1
2	rhodopsin	2

Entities:

Entity 1, Arr67_77 11 residues - Formula weight is not available

1

TYR

GLY

GLN

GLU

ASP

ILE

ASP

VAL

MET

GLY

2

LEU

Entity 2, rhodopsin - Formula weight is not available

Samples:

sample_1: Arr67_77 2 mM; rhodopsin 50 uM; sodium phosphate 10 mM; potassium chloride 20 mM; D2O 10 v/v; H2O 90 v/v

sample_2: Arr67_77 1 mM; sodium phosphate 10 mM; potassium chloride 20 mM; D2O 10 v/v; H2O 90 v/v

sample_conditions_1: pH: 6.6; pressure: 1.0 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
T-ROESY	sample_2	isotropic	sample_conditions_1

Software:

VNMR, Varian - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz