BMRB Entry 19504
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19504
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Citation: Kier, Brandon; Sheffler, William; Baker, David. "Covalent Assembly of Homooligomeric Proteins Using Structure-templating Hubs" Biochemistry ., .-..
Assembly members:
entity, polymer, 38 residues, 4599.372 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: RWLCIWLSDQTLEDLEKMAR
REGLSKSEMINVALQHYK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 294 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | baa38_1 | 1 |
| 2 | baa38_2 | 1 |
Entities:
Entity 1, baa38_1 38 residues - 4599.372 Da.
| 1 | ARG | TRP | LEU | CYS | ILE | TRP | LEU | SER | ASP | GLN | ||||
| 2 | THR | LEU | GLU | ASP | LEU | GLU | LYS | MET | ALA | ARG | ||||
| 3 | ARG | GLU | GLY | LEU | SER | LYS | SER | GLU | MET | ILE | ||||
| 4 | ASN | VAL | ALA | LEU | GLN | HIS | TYR | LYS |
Samples:
sample_1: baa38 mM; sodium phosphate 50 mM; DSS 0.5 mM; H20 90%; D2O 10%
sample_conditions_1: ionic strength: 0.11 M; pH: 5; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Foldit, David Baker et al, University of Washington - design
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
SPARKY, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz