BMRB Entry 19382

Title:
Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'   PubMed: 24692659
Deposition date:
2013-07-22
Original release date:
2014-05-06
Authors:
Moursy, Ahmed; Allain, Frederic; Clery, Antoine
Citation:

Citation: Moursy, Ahmed; Allain, Frederic H-T; Clery, Antoine. "Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation."  Nucleic Acids Res. ., .-. (2014).

Assembly members:

Assembly members:
hnRNP_G_RRM, polymer, 95 residues, 10413.906 Da.
RNA_AUCAAA, polymer, 6 residues, 1883.228 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts97
1H chemical shifts685

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNP G RRM1
2RNA (5'-R(*AP*UP*CP*AP*AP*A)-3')2

Entities:

Entity 1, hnRNP G RRM 95 residues - 10413.906 Da.

1   METVALGLUALAASPARGPROGLYLYSLEU
2   PHEILEGLYGLYLEUASNTHRGLUTHRASN
3   GLULYSALALEUGLUALAVALPHEGLYLYS
4   TYRGLYARGILEVALGLUVALLEULEUMET
5   LYSASPARGGLUTHRASNLYSSERARGGLY
6   PHEALAPHEVALTHRPHEGLUSERPROALA
7   ASPALALYSASPALAALAARGASPMETASN
8   GLYLYSSERLEUASPGLYLYSALAILELYS
9   VALGLUGLNALATHRLYSPROSERPHEGLU
10   SERGLYARGARGGLY

Entity 2, RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 6 residues - 1883.228 Da.

1   AUCAAA

Samples:

15N_H2O: hnRNP_G_RRM, [U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; H2O 90%; D2O 10%; NaCl 50 mM; NaH2PO4 20 mM

15N_D2O: hnRNP_G_RRM, [U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; D2O 100%; NaCl 50 mM; NaH2PO4 20 mM

13C_H2O: hnRNP_G_RRM, [U-100% 13C; U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; H2O 90%; D2O 10%; NaCl 50 mM; NaH2PO4 20 mM

13C_D2O: hnRNP_G_RRM, [U-100% 13C; U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; D2O 100%; NaCl 50 mM; NaH2PO4 20 mM

sample_conditions_1: ionic strength: 70 mM; pH: 5.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_H2Oisotropicsample_conditions_1
2D 1H-13C HSQC aliphatic13C_H2Oisotropicsample_conditions_1
2D 1H-13C HSQC aromatic13C_H2Oisotropicsample_conditions_1
2D 1H-1H TOCSY15N_D2Oisotropicsample_conditions_1
2D 1H-1H NOESY15N_D2Oisotropicsample_conditions_1
3D CBCA(CO)NH13C_H2Oisotropicsample_conditions_1
3D HNCA13C_H2Oisotropicsample_conditions_1
3D HNCACB13C_H2Oisotropicsample_conditions_1
3D HCCH-TOCSY13C_H2Oisotropicsample_conditions_1
3D 1H-13C NOESY aliphatic13C_H2Oisotropicsample_conditions_1
3D 1H-13C NOESY aromatic13C_H2Oisotropicsample_conditions_1
3D 1H-15N NOESY15N_H2Oisotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 19792
PDB
DBJ BAC31099 BAE00972 BAE22448 BAE37998 BAF62396
EMBL CAA80599 CAB51361 CAB51362 CAG31684 CAI46148
GB AAH03710 AAH06550 AAH07435 AAH11441 AAH12942
PIR S41766
REF NP_001020834 NP_001073196 NP_001100890 NP_001128678 NP_001156008
SP A5A6M3 D4AE41 P38159 P84586 Q4R7F0
TPG DAA13328 DAA13329
AlphaFold A5A6M3

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts