BMRB Entry 19230
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PDB ID: 2m86
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19230
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ji, Yanbin; Majumder, Subhabrata; Millard, Melissa; Borra, Radhika; Bi, Tao; Elnagar, Ahmed; Neamati, Nouri; Shekhtman, Alexander; Camarero, Julio. "In Vivo Activation of the p53 Tumor Suppressor Pathway by an Engineered Cyclotide." J. Am. Chem. Soc. 135, 11623-11633 (2013).
PubMed: 23848581
Assembly members:
Hdm2, polymer, 51 residues, 5291.016 Da.
MCo-PMI, polymer, 83 residues, 9814.753 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTXB1
Entity Sequences (FASTA):
Hdm2: SGSGASKAPTSFAEYWNLLS
AGGVCPKILQRCRRDSDCPG
ACICRGNGYCG
MCo-PMI: LVRPKPLLLKLLKSVGAQKD
TYTMKEVLFYLGQYIMTKRL
YDEKQQHIVYCSNDLLGDLF
GVPSFSVKEHRKIYTMIYRN
LVV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 147 |
| 15N chemical shifts | 121 |
| 1H chemical shifts | 581 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hdm2 | 1 |
| 2 | MCo-PMI | 2 |
Entities:
Entity 1, Hdm2 51 residues - 5291.016 Da.
This is an engineered cyclotide based on MCoTI-I., cyclic-pseudo-peptide
| 1 | SER | GLY | SER | GLY | ALA | SER | LYS | ALA | PRO | THR | ||||
| 2 | SER | PHE | ALA | GLU | TYR | TRP | ASN | LEU | LEU | SER | ||||
| 3 | ALA | GLY | GLY | VAL | CYS | PRO | LYS | ILE | LEU | GLN | ||||
| 4 | ARG | CYS | ARG | ARG | ASP | SER | ASP | CYS | PRO | GLY | ||||
| 5 | ALA | CYS | ILE | CYS | ARG | GLY | ASN | GLY | TYR | CYS | ||||
| 6 | GLY |
Entity 2, MCo-PMI 83 residues - 9814.753 Da.
| 1 | LEU | VAL | ARG | PRO | LYS | PRO | LEU | LEU | LEU | LYS | ||||
| 2 | LEU | LEU | LYS | SER | VAL | GLY | ALA | GLN | LYS | ASP | ||||
| 3 | THR | TYR | THR | MET | LYS | GLU | VAL | LEU | PHE | TYR | ||||
| 4 | LEU | GLY | GLN | TYR | ILE | MET | THR | LYS | ARG | LEU | ||||
| 5 | TYR | ASP | GLU | LYS | GLN | GLN | HIS | ILE | VAL | TYR | ||||
| 6 | CYS | SER | ASN | ASP | LEU | LEU | GLY | ASP | LEU | PHE | ||||
| 7 | GLY | VAL | PRO | SER | PHE | SER | VAL | LYS | GLU | HIS | ||||
| 8 | ARG | LYS | ILE | TYR | THR | MET | ILE | TYR | ARG | ASN | ||||
| 9 | LEU | VAL | VAL |
Related Database Links:
| PDB | 2M86 1RV1 1T4E 1T4F 1YCR 1Z1M 2AXI 2GV2 2LZG 2RUH 3EQS 3G03 3IUX 3IWY 3JZK 3JZR 3JZS 3LBK 3LBL 3LNJ 3LNZ 3TJ2 3TPX 3TU1 3V3B 3VBG 3VZV 3W69 4DIJ 4ERE 4ERF 4HBM 4HFZ 4HG7 4JV7 4JV9 4JVE 4JVR 4JWR 4MDN 4MDQ 4OAS 4OBA 4OCC 4ODE 4ODF 4OGN 4OGT 4OGV 4OQ3 4QO4 4UMN 4WT2 |
| BMRB | 15945 18755 2410 6612 |
| DBJ | BAB11975 BAC78209 BAF83030 BAJ17752 |
| EMBL | CAA78055 CAD23251 CAD36959 CAH89564 CAP16708 |
| GB | AAA60568 AAF28866 AAF67833 AAG42840 AAG42841 |
| PRF | 1814460A |
| REF | NP_001003103 NP_001009346 NP_001092577 NP_001098773 NP_001124685 |
| SP | P56950 P56951 Q00987 Q7YRZ8 |
| TPG | DAA29805 |
| AlphaFold | P56950 P56951 Q00987 Q7YRZ8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks