BMRB Entry 18662

Name: Solution NMR structure of SH3 domain of growth arrest-specific protein 7 (GAS7)(fragment 1-60)from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8574A
Published: 2012-10-09

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PDB ID: 2lx7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18662
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of SH3 domain of growth arrest-specific protein 7 (GAS7)(fragment 1-60)from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8574A

Deposition date:

2012-08-15

Original release date:

2012-10-09

Authors:

Yang, Yunhuang; Ramelot, Theresa; Dan, Lee; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Yang, Yunhuang; Ramelot, Theresa; Dan, Lee; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of SH3 domain of growth arrest-specific protein 7 (GAS7) (fragment 1-60)from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8574A" To be published ., .-..

Assembly members:

Assembly members:
SH3_domain_of_GAS7, polymer, 60 residues, 6718.633 Da.

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: HR8574A-1-60-AV6HT.2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3_domain_of_GAS7: MSGARCRTLYPFSGERHGQG LRFAAGELITLLQVPDGGWW EGEKEDGLRGWFPASYVQLL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	64
1H chemical shifts	413

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3_domain_of_GAS7	1

Entities:

Entity 1, SH3_domain_of_GAS7 60 residues - 6718.633 Da.

1	MET	SER	GLY	ALA	ARG	CYS	ARG	THR	LEU	TYR
2	PRO	PHE	SER	GLY	GLU	ARG	HIS	GLY	GLN	GLY
3	LEU	ARG	PHE	ALA	ALA	GLY	GLU	LEU	ILE	THR
4	LEU	LEU	GLN	VAL	PRO	ASP	GLY	GLY	TRP	TRP
5	GLU	GLY	GLU	LYS	GLU	ASP	GLY	LEU	ARG	GLY
6	TRP	PHE	PRO	ALA	SER	TYR	VAL	GLN	LEU	LEU

Samples:

NC_sample: SH3_domain_of_GAS7, [U-100% 13C; U-100% 15N], 0.26 ± 0.02 mM; NaN3 0.02 ± 0.001 %; DTT 10 ± 0.05 mM; CaCL2 5 ± 0.025 mM; NaCL 100 ± 0.5 mM; Proteinase Inhibitors 1 x; MES pH 6.5 20 ± 0.01 mM; D2O 10 ± 0.005 %; DSS 50 ± 0.025 uM; H2O 90 ± 0.005 %

NC5_sample: SH3_domain_of_GAS7, [U-10% 13C; U-100% 15N], 0.45 ± 0.02 mM; NaN3 0.02 ± 0.001 %; DTT 10 ± 0.05 mM; CaCL2 5 ± 0.025 mM; NaCL 100 ± 0.5 mM; Proteinase Inhibitors 1 x; MES pH 6.5 20 ± 0.01 mM; D2O 10 ± 0.005 %; DSS 50 ± 0.025 uM; H2O 90 ± 0.005 %

NC_sample_in_D2O: SH3_domain_of_GAS7, [U-100% 13C; U-100% 15N], 0.26 ± 0.02 mM; NaN3 0.02 ± 0.001 %; DTT 10 ± 0.05 mM; CaCL2 5 ± 0.025 mM; NaCL 100 ± 0.5 mM; Proteinase Inhibitors 1 x; MES pH 6.5 20 ± 0.01 mM; DSS 50 ± 0.025 uM; D2O 100 ± 0.005 %

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC	NC_sample	isotropic	sample_conditions_1
3D HNCO	NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HNCACB	NC_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	NC_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	NC_sample	isotropic	sample_conditions_1
4D CC-NOESY	NC_sample_in_D2O	isotropic	sample_conditions_1
3D HNCA	NC_sample	isotropic	sample_conditions_1
3D HN(CO)CA	NC_sample	isotropic	sample_conditions_1
3D H(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D C(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC_sample	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample	isotropic	sample_conditions_1
3D HNHA	NC_sample	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC-arom	NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC-Histidine	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC - NC5	NC5_sample	isotropic	sample_conditions_1

PDB	2LX7
DBJ	BAC21639 BAE89369
GB	AAP92798 EAW90011 ELK03871 ELW53078
REF	NP_958839 XP_002747958 XP_002827074 XP_003274725 XP_003315707
SP	O60861
AlphaFold	O60861

BMRB Entry 18662

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	SER	GLY	ALA	ARG	CYS	ARG	THR	LEU	TYR
2	PRO	PHE	SER	GLY	GLU	ARG	HIS	GLY	GLN	GLY
3	LEU	ARG	PHE	ALA	ALA	GLY	GLU	LEU	ILE	THR
4	LEU	LEU	GLN	VAL	PRO	ASP	GLY	GLY	TRP	TRP
5	GLU	GLY	GLU	LYS	GLU	ASP	GLY	LEU	ARG	GLY
6	TRP	PHE	PRO	ALA	SER	TYR	VAL	GLN	LEU	LEU

1	MET	SER	GLY	ALA	ARG	CYS	ARG	THR	LEU	TYR
2	PRO	PHE	SER	GLY	GLU	ARG	HIS	GLY	GLN	GLY
3	LEU	ARG	PHE	ALA	ALA	GLY	GLU	LEU	ILE	THR
4	LEU	LEU	GLN	VAL	PRO	ASP	GLY	GLY	TRP	TRP
5	GLU	GLY	GLU	LYS	GLU	ASP	GLY	LEU	ARG	GLY
6	TRP	PHE	PRO	ALA	SER	TYR	VAL	GLN	LEU	LEU

1	MET	SER	GLY	ALA	ARG	CYS	ARG	THR	LEU	TYR
2	PRO	PHE	SER	GLY	GLU	ARG	HIS	GLY	GLN	GLY
3	LEU	ARG	PHE	ALA	ALA	GLY	GLU	LEU	ILE	THR
4	LEU	LEU	GLN	VAL	PRO	ASP	GLY	GLY	TRP	TRP
5	GLU	GLY	GLU	LYS	GLU	ASP	GLY	LEU	ARG	GLY
6	TRP	PHE	PRO	ALA	SER	TYR	VAL	GLN	LEU	LEU