BMRB Entry 17916

Name: Backbone assignments for the C-terminal domain of yeast Tfg2
Published: 2011-12-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17916

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments for the C-terminal domain of yeast Tfg2

Deposition date:

2011-09-06

Original release date:

2011-12-14

Authors:

Kilpatrick, Adina; Koharudin, Leonardus; Gronenborn, Angela

Citation:

Citation: Kilpatrick, Adina; Koharudin, Leonardus; Calero, Guillermo; Gronenborn, Angela. "Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2." Proteins ., .-. (2011).
PubMed: 22095626

Assembly members:

Assembly members:
Tfg2, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tfg2: MASKSNIKSIRMPKKEILDY LFKLFDEYDYWSLKGLKERT RQPEAHLKECLDKVATLVKK GPYAFKYTLRPEYKKLKEEE RKATLGELADEQT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	270
15N chemical shifts	89
1H chemical shifts	176

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tfg2	1

Entities:

Entity 1, Tfg2 93 residues - Formula weight is not available

Residues 1-3 represent a non-native affinity tag.

1

MET

ALA

SER

LYS

SER

ASN

ILE

LYS

SER

ILE

2

ARG

MET

PRO

LYS

GLU

ILE

LEU

ASP

TYR

3

LEU

PHE

LYS

LEU

PHE

ASP

GLU

TYR

ASP

TYR

4

TRP

SER

LEU

LYS

GLY

LEU

LYS

GLU

ARG

THR

5

ARG

GLN

PRO

GLU

ALA

HIS

LEU

LYS

GLU

CYS

6

LEU

ASP

LYS

VAL

ALA

THR

LEU

VAL

LYS

7

GLY

PRO

TYR

ALA

PHE

LYS

TYR

THR

LEU

ARG

8

PRO

GLU

TYR

LYS

LEU

LYS

GLU

9

ARG

LYS

ALA

THR

LEU

GLY

GLU

LEU

ALA

ASP

10

GLU

GLN

THR

Samples:

sample_1: Tfg2, [U-98% 13C; U-98% 15N], 1 – 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

PDB	4V1N 4V1O
DBJ	GAA23391
EMBL	CAA96988 CAY79765
GB	AAA61642 AAT92890 AHY79373 AJP38791 AJR76111
REF	NP_011519
SP	P41896
TPG	DAA08103
AlphaFold	P41896