BMRB Entry 17916

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17916

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Title:
Backbone assignments for the C-terminal domain of yeast Tfg2
Deposition date:
2011-09-06
Original release date:
2011-12-14
Authors:
Kilpatrick, Adina; Koharudin, Leonardus; Gronenborn, Angela
Citation:

Citation: Kilpatrick, Adina; Koharudin, Leonardus; Calero, Guillermo; Gronenborn, Angela. "Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2."  Proteins ., .-. (2011).
PubMed: 22095626

Assembly members:

Assembly members:
Tfg2, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tfg2: MASKSNIKSIRMPKKEILDY LFKLFDEYDYWSLKGLKERT RQPEAHLKECLDKVATLVKK GPYAFKYTLRPEYKKLKEEE RKATLGELADEQT

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts89
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tfg21

Entities:

Entity 1, Tfg2 93 residues - Formula weight is not available

Residues 1-3 represent a non-native affinity tag.

1   METALASERLYSSERASNILELYSSERILE
2   ARGMETPROLYSLYSGLUILELEUASPTYR
3   LEUPHELYSLEUPHEASPGLUTYRASPTYR
4   TRPSERLEULYSGLYLEULYSGLUARGTHR
5   ARGGLNPROGLUALAHISLEULYSGLUCYS
6   LEUASPLYSVALALATHRLEUVALLYSLYS
7   GLYPROTYRALAPHELYSTYRTHRLEUARG
8   PROGLUTYRLYSLYSLEULYSGLUGLUGLU
9   ARGLYSALATHRLEUGLYGLULEUALAASP
10   GLUGLNTHR

Related Database Links:

PDB 4V1N 4V1O
DBJ GAA23391
EMBL CAA96988 CAY79765
GB AAA61642 AAT92890 AHY79373 AJP38791 AJR76111
REF NP_011519
SP P41896
TPG DAA08103
AlphaFold P41896

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks