BMRB Entry 17896

Title:
NOE-based 3D structure of the monomeric intermediate of CylR2 at 262K (-11 Celsius degrees)
Deposition date:
2011-08-29
Original release date:
2013-02-15
Authors:
Jaremko, Mariusz; Jaremko, Lukasz; Kim, Hai-Young; Cho, Min-Kyu; Schwieters, Charles; Giller, Karin; Becker, Stefan; Zweckstetter, Markus
Citation:

Citation: Jaremko, Mariusz; Jaremko, Lukasz; Kim, Hai-Young; Cho, Min-Kyu; Schwieters, Charles; Giller, Karin; Becker, Stefan; Zweckstetter, Markus. "Cold denaturation of a protein dimer monitored at atomic resolution."  Nat. Chem. Biol. 9, 264-270 (2013).
PubMed: 23396077

Assembly members:

Assembly members:
CylR2, polymer, 66 residues, 7725.070 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET32A

Entity Sequences (FASTA):

Study: Cold denaturation of the CylR2 homodimer monitored at atomic resolution.

25 °C -3 °C -7 °C Dimer -7 °C Monomer -11 °C -14 °C -16 °C
17892178931789417895178961789717898
2lyj2lyk2lyl2lyp2lyq2lyr2lys

25°C -16°C

Adapted by permission from Macmillan Publishers Ltd: Nature Chemical Biology, copyright 2013

Molecular graphics and analyses were performed with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311).
UCSF Chimera--a visualization system for exploratory research and analysis. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE. J Comput Chem. 2004 Oct;25(13):1605-12.
JSmol: an open-source HTML5 viewer for chemical structures in 3D.

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts69
1H chemical shifts419

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CylR21

Entities:

Entity 1, CylR2 66 residues - 7725.070 Da.

1   METILEILEASNASNLEULYSLEUILEARG
2   GLULYSLYSLYSILESERGLNSERGLULEU
3   ALAALALEULEUGLUVALSERARGGLNTHR
4   ILEASNGLYILEGLULYSASNLYSTYRASN
5   PROSERLEUGLNLEUALALEULYSILEALA
6   TYRTYRLEUASNTHRPROLEUGLUASPILE
7   PHEGLNTRPGLNPROGLU

Related Database Links:

BMRB 17892 17893 17894 17895 17897 17898
PDB 1UTX 2GZU 2LYJ 2LYK 2LYL 2LYP 2LYQ 2LYR 2LYS 2XI8 2XIU 2XJ3
GB AAL59476 AAL60140 AAM75247 AAO80372 AIL03173
REF NP_814301 WP_002358483 WP_002370931 WP_010773902 WP_048951311

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks