BMRB Entry 17783

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PDB ID: 2lfv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17783
MolProbity Validation Chart

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Title:
Solution Structure of the SPOR domain from E. coli DamX
Deposition date:
2011-07-15
Original release date:
2012-07-17
Authors:
Williams, Kyle; Arends, S.J.; Popham, David; Fowler, C.; Weiss, David
Citation:

Citation: Williams, Kyle; Yahashiri, Atsushi; Arends, S.J.; Popham, David; Fowler, C.; Weiss, David. "Nuclear Magnetic Resonance Solution Structure of the Peptidoglycan-Binding SPOR Domain from Escherichia coli DamX: Insights into Septal Localization"  Biochemistry 52, 627-639 (2013).
PubMed: 23290046

Assembly members:

Assembly members:
DamX_SPOR_domain_polypeptide, polymer, 106 residues, 11876.272 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE80L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DamX_SPOR_domain_polypeptide: MRGSHHHHHHGSNNNGSLKS APSSHYTLQLSSSSNYDNLN GWAKKENLKNYVVYETTRNG QPWYVLVSGVYASKEEAKKA VSTLPADVQAKNPWAKPLRQ VQADLK

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts114
1H chemical shifts994
heteronuclear NOE values94
T1 relaxation values94
T2 relaxation values94

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DamX SPOR domain1

Entities:

Entity 1, DamX SPOR domain 106 residues - 11876.272 Da.

first residue number is 323 residues 323-337 constitute an added affinity tag and linker the actual SPOR domain starts at residue 344

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERASNASNASNGLYSERLEULYSSER
3   ALAPROSERSERHISTYRTHRLEUGLNLEU
4   SERSERSERSERASNTYRASPASNLEUASN
5   GLYTRPALALYSLYSGLUASNLEULYSASN
6   TYRVALVALTYRGLUTHRTHRARGASNGLY
7   GLNPROTRPTYRVALLEUVALSERGLYVAL
8   TYRALASERLYSGLUGLUALALYSLYSALA
9   VALSERTHRLEUPROALAASPVALGLNALA
10   LYSASNPROTRPALALYSPROLEUARGGLN
11   VALGLNALAASPLEULYS

Related Database Links:

PDB 2LFV
DBJ BAB37653 BAE77903 BAG79173 BAI27646 BAI32816
EMBL CAA33253 CAP77835 CAQ33709 CAR00327 CAR04988
GB AAA58185 AAC76413 AAG58488 AAN44868 AAN82596
REF NP_312257 NP_417847 NP_709161 WP_000343127 WP_000343163
SP P11557 Q8X826
AlphaFold P11557 Q8X826

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