BMRB Entry 17246
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PDB ID: 2l4o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17246
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gentile, Maria Antonietta; Melchiorre, Sara; Emolo, Carla; Moschioni, Monica; Gianfaldoni, Claudia; Pancotto, Laura; Ferlenghi, Ilaria; Scarselli, Maria; Pansegrau, Werner; Veggi, Daniele; Merola, Marcello; Cantini, Francesca; Ruggiero, Paolo; Banci, Lucia; Masignani, Vega. "Structural and Functional Characterization of the Streptococcus pneumoniae RrgB Pilus Backbone D1 Domain." J. Biol. Chem. 286, 14588-14597 (2011).
PubMed: 21367860
Assembly members:
DOMAIN_OF_A_CELL_WALL_SURFACE_ANCHOR_FAMILY_PROTEIN, polymer, 181 residues, 18545.027 Da.
Natural source: Common Name: Streptococcus pneumoniae Taxonomy ID: 1313 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus pneumoniae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21b+
Entity Sequences (FASTA):
DOMAIN_OF_A_CELL_WALL_SURFACE_ANCHOR_FAMILY_PROTEIN: METASAATVFAAGTTTTSVT
VHKLLATDGDMDKIANELET
GNYAGNKVGVLPANAKEIAG
VMFVWTNTNNEIIDENGQTL
GVNIDPQTFKLSGAMPATAM
KKLTEAEGAKFNTANLPAAK
YKIYEIHSLSTYVGEDGATL
TGSKAVPIEIELPLNDVVDA
HVYPKNTEAKPKILEHHHHH
H
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 660 |
| 15N chemical shifts | 172 |
| 1H chemical shifts | 1168 |
| heteronuclear NOE values | 150 |
| order parameters | 150 |
| T1 relaxation values | 150 |
| T2 relaxation values | 150 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CELL_WALL_SURFACE_ANCHOR | 1 |
Entities:
Entity 1, CELL_WALL_SURFACE_ANCHOR 181 residues - 18545.027 Da.
The C-terminal His-tag is removed from the pdb
| 1 | MET | GLU | THR | ALA | SER | ALA | ALA | THR | VAL | PHE | ||||
| 2 | ALA | ALA | GLY | THR | THR | THR | THR | SER | VAL | THR | ||||
| 3 | VAL | HIS | LYS | LEU | LEU | ALA | THR | ASP | GLY | ASP | ||||
| 4 | MET | ASP | LYS | ILE | ALA | ASN | GLU | LEU | GLU | THR | ||||
| 5 | GLY | ASN | TYR | ALA | GLY | ASN | LYS | VAL | GLY | VAL | ||||
| 6 | LEU | PRO | ALA | ASN | ALA | LYS | GLU | ILE | ALA | GLY | ||||
| 7 | VAL | MET | PHE | VAL | TRP | THR | ASN | THR | ASN | ASN | ||||
| 8 | GLU | ILE | ILE | ASP | GLU | ASN | GLY | GLN | THR | LEU | ||||
| 9 | GLY | VAL | ASN | ILE | ASP | PRO | GLN | THR | PHE | LYS | ||||
| 10 | LEU | SER | GLY | ALA | MET | PRO | ALA | THR | ALA | MET | ||||
| 11 | LYS | LYS | LEU | THR | GLU | ALA | GLU | GLY | ALA | LYS | ||||
| 12 | PHE | ASN | THR | ALA | ASN | LEU | PRO | ALA | ALA | LYS | ||||
| 13 | TYR | LYS | ILE | TYR | GLU | ILE | HIS | SER | LEU | SER | ||||
| 14 | THR | TYR | VAL | GLY | GLU | ASP | GLY | ALA | THR | LEU | ||||
| 15 | THR | GLY | SER | LYS | ALA | VAL | PRO | ILE | GLU | ILE | ||||
| 16 | GLU | LEU | PRO | LEU | ASN | ASP | VAL | VAL | ASP | ALA | ||||
| 17 | HIS | VAL | TYR | PRO | LYS | ASN | THR | GLU | ALA | LYS | ||||
| 18 | PRO | LYS | ILE | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 19 | HIS |
Related Database Links:
| PDB | 2L4O 2Y1V 3RPK |
| EMBL | CEO70617 CEV45859 CEV80893 CEV87170 CEV93515 |
| GB | AAK74623 ABS82086 ABS82156 ACO23362 ADI68988 |
| REF | WP_000836216 WP_000836217 WP_000836218 WP_000836219 WP_000836220 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks