BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17190

Title: Complex Pfu Rpp30   PubMed: 22162665

Deposition date: 2010-09-13 Original release date: 2012-05-22

Authors: Crowe, Brandon; Foster, Mark

Citation: Crowe, Brandon; Bohlen, Christopher; Wilson, Ross; Gopalan, Venkat; Foster, Mark. "Assembly of the complex between archaeal RNase P proteins RPP30 and Pop5."  Archaea 2011, 891531-891531 (2011).

Assembly members:
Rpp30, polymer, 217 residues, Formula weight is not available
Pop5, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET33b

Entity Sequences (FASTA):
Rpp30: GEFMAGGRNGVKFVEMDIRS REAYELAEEWFDDVVFSYEI PPGVLDKERLKEIKKEYGNV AITLINPKPSLVKEAVQRFK QNYLIYVESSDLRVVRYSIE RGVDAVISPWANRKDQGIDH VLARMMNKRGVALGFSLRPL LHQNPYERANALKFMRKAWT LVNKYKVPRFISSSAKGKFQ VRGVKELISLGIAIGMEEVQ AKASLSFYPLGILERLK
Pop5: MSERPKTLPPTLRDKKRYIA FKVISENQFNKDEIKEAIWN ACLRTLGELGTAKAKPWLIK FDETTQTGIIRSDRNHVYDV IFSLTLVSDINGNKAIIKVL GVSGTIKRLKRKFLSQFGWR

Data sets:
Data typeCount
13C chemical shifts465
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rpp301
2Pop52

Entities:

Entity 1, Rpp30 217 residues - Formula weight is not available

Pfu Rpp30 native with 3 residues as artifact from cleavage of affinity tag.

1   GLYGLUPHEMETALAGLYGLYARGASNGLY
2   VALLYSPHEVALGLUMETASPILEARGSER
3   ARGGLUALATYRGLULEUALAGLUGLUTRP
4   PHEASPASPVALVALPHESERTYRGLUILE
5   PROPROGLYVALLEUASPLYSGLUARGLEU
6   LYSGLUILELYSLYSGLUTYRGLYASNVAL
7   ALAILETHRLEUILEASNPROLYSPROSER
8   LEUVALLYSGLUALAVALGLNARGPHELYS
9   GLNASNTYRLEUILETYRVALGLUSERSER
10   ASPLEUARGVALVALARGTYRSERILEGLU
11   ARGGLYVALASPALAVALILESERPROTRP
12   ALAASNARGLYSASPGLNGLYILEASPHIS
13   VALLEUALAARGMETMETASNLYSARGGLY
14   VALALALEUGLYPHESERLEUARGPROLEU
15   LEUHISGLNASNPROTYRGLUARGALAASN
16   ALALEULYSPHEMETARGLYSALATRPTHR
17   LEUVALASNLYSTYRLYSVALPROARGPHE
18   ILESERSERSERALALYSGLYLYSPHEGLN
19   VALARGGLYVALLYSGLULEUILESERLEU
20   GLYILEALAILEGLYMETGLUGLUVALGLN
21   ALALYSALASERLEUSERPHETYRPROLEU
22   GLYILELEUGLUARGLEULYS

Entity 2, Pop5 120 residues - Formula weight is not available

Pop5 from Pfu with C72S mutation

1   METSERGLUARGPROLYSTHRLEUPROPRO
2   THRLEUARGASPLYSLYSARGTYRILEALA
3   PHELYSVALILESERGLUASNGLNPHEASN
4   LYSASPGLUILELYSGLUALAILETRPASN
5   ALACYSLEUARGTHRLEUGLYGLULEUGLY
6   THRALALYSALALYSPROTRPLEUILELYS
7   PHEASPGLUTHRTHRGLNTHRGLYILEILE
8   ARGSERASPARGASNHISVALTYRASPVAL
9   ILEPHESERLEUTHRLEUVALSERASPILE
10   ASNGLYASNLYSALAILEILELYSVALLEU
11   GLYVALSERGLYTHRILELYSARGLEULYS
12   ARGLYSPHELEUSERGLNPHEGLYTRPARG

Samples:

sample_1: Rpp30, [U-100% 13C; U-100% 15N], 450 uM; Pop5 450 uM; DSS 66.67 uM; D2O 5%; H2O 95%; TRIS 10 mM; Sodium Azide 0.02%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 17189 6917
GB AAL82038 AFN04726 AAL81502 AFN04159
REF WP_011013054 NP_579107 WP_011012525 YP_006492451
SP Q8TZS0 Q8U151
PDB

Download simulated HSQC data in one of the following formats:
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