BMRB Entry 6917

Name: Backbone assignments of Pyrococcus furiosus Pop5 (PF1378), an archaeal RNase P protein.
Published: 2006-01-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6917

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of Pyrococcus furiosus Pop5 (PF1378), an archaeal RNase P protein.

Deposition date:

2005-12-05

Original release date:

2006-01-23

Authors:

Wilson, Ross; Foster, Mark

Citation:

Citation: Wilson, Ross; Bohlen, C.; Foster, Mark; Bell, C. "Structure of Pfu Pop5, an archaeal RNase P protein" Proc. Natl. Acad. Sci. U.S.A. 103, 873-878 (2006).
PubMed: 16418270

Assembly members:

Assembly members:
Pfu pop5, polymer, 120 residues, 13708 Da.

Natural source:

Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pfu pop5: MSERPKTLPPTLRDKKRYIA FKVISENQFNKDEIKEAIWN ACLRTLGELGTAKAKPWLIK FDETTQTGIIRSDRNHVYDV IFSLTLVSDINGNKAIIKVL GVSGTIKRLKRKFLSQFGWR

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	306
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pop5 monomer	1

Entities:

Entity 1, pop5 monomer 120 residues - 13708 Da.

1	MET	SER	GLU	ARG	PRO	LYS	THR	LEU	PRO	PRO
2	THR	LEU	ARG	ASP	LYS	LYS	ARG	TYR	ILE	ALA
3	PHE	LYS	VAL	ILE	SER	GLU	ASN	GLN	PHE	ASN
4	LYS	ASP	GLU	ILE	LYS	GLU	ALA	ILE	TRP	ASN
5	ALA	CYS	LEU	ARG	THR	LEU	GLY	GLU	LEU	GLY
6	THR	ALA	LYS	ALA	LYS	PRO	TRP	LEU	ILE	LYS
7	PHE	ASP	GLU	THR	THR	GLN	THR	GLY	ILE	ILE
8	ARG	SER	ASP	ARG	ASN	HIS	VAL	TYR	ASP	VAL
9	ILE	PHE	SER	LEU	THR	LEU	VAL	SER	ASP	ILE
10	ASN	GLY	ASN	LYS	ALA	ILE	ILE	LYS	VAL	LEU
11	GLY	VAL	SER	GLY	THR	ILE	LYS	ARG	LEU	LYS
12	ARG	LYS	PHE	LEU	SER	GLN	PHE	GLY	TRP	ARG

Samples:

sample_1: Pfu pop5, [U-95% 13C; U-95% 15N], 0.3 mM; Sodium Acetate 10 mM; Potassium Chloride 0.5 mM; Sodium Azide 0.05 mM

conditions_1: pH: 5.0; temperature: 328 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	conditions_1
1H1513C HNCO	sample_1	not available	conditions_1
1H1513C HNCA	sample_1	not available	conditions_1
1H1513C HNCACB	sample_1	not available	conditions_1
1H1513C CBCACONH	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance DRX 600 MHz

BMRB	17190
PDB	2AV5
GB	AAL81502 AFN04159
REF	NP_579107 WP_011012525 YP_006492451
SP	Q8U151
AlphaFold	Q8U151

1	MET	SER	GLU	ARG	PRO	LYS	THR	LEU	PRO	PRO
2	THR	LEU	ARG	ASP	LYS	LYS	ARG	TYR	ILE	ALA
3	PHE	LYS	VAL	ILE	SER	GLU	ASN	GLN	PHE	ASN
4	LYS	ASP	GLU	ILE	LYS	GLU	ALA	ILE	TRP	ASN
5	ALA	CYS	LEU	ARG	THR	LEU	GLY	GLU	LEU	GLY
6	THR	ALA	LYS	ALA	LYS	PRO	TRP	LEU	ILE	LYS
7	PHE	ASP	GLU	THR	THR	GLN	THR	GLY	ILE	ILE
8	ARG	SER	ASP	ARG	ASN	HIS	VAL	TYR	ASP	VAL
9	ILE	PHE	SER	LEU	THR	LEU	VAL	SER	ASP	ILE
10	ASN	GLY	ASN	LYS	ALA	ILE	ILE	LYS	VAL	LEU
11	GLY	VAL	SER	GLY	THR	ILE	LYS	ARG	LEU	LYS
12	ARG	LYS	PHE	LEU	SER	GLN	PHE	GLY	TRP	ARG

1	MET	SER	GLU	ARG	PRO	LYS	THR	LEU	PRO	PRO
2	THR	LEU	ARG	ASP	LYS	LYS	ARG	TYR	ILE	ALA
3	PHE	LYS	VAL	ILE	SER	GLU	ASN	GLN	PHE	ASN
4	LYS	ASP	GLU	ILE	LYS	GLU	ALA	ILE	TRP	ASN
5	ALA	CYS	LEU	ARG	THR	LEU	GLY	GLU	LEU	GLY
6	THR	ALA	LYS	ALA	LYS	PRO	TRP	LEU	ILE	LYS
7	PHE	ASP	GLU	THR	THR	GLN	THR	GLY	ILE	ILE
8	ARG	SER	ASP	ARG	ASN	HIS	VAL	TYR	ASP	VAL
9	ILE	PHE	SER	LEU	THR	LEU	VAL	SER	ASP	ILE
10	ASN	GLY	ASN	LYS	ALA	ILE	ILE	LYS	VAL	LEU
11	GLY	VAL	SER	GLY	THR	ILE	LYS	ARG	LEU	LYS
12	ARG	LYS	PHE	LEU	SER	GLN	PHE	GLY	TRP	ARG