BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17189

Title: Pfu Rpp30 Free   PubMed: 22162665

Deposition date: 2010-09-13 Original release date: 2012-05-22

Authors: Crowe, Brandon

Citation: Crowe, Brandon; Bohlen, Christopher; Wilson, Ross; Gopalan, Venkat; Foster, Mark. "Assembly of the complex between archaeal RNase P proteins RPP30 and Pop5."  Archaea 2011, 891531-891531 (2011).

Assembly members:
Rpp30, polymer, 217 residues, Formula weight is not available

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET33b

Entity Sequences (FASTA):
Rpp30: GEFMAGGRNGVKFVEMDIRS REAYELAEEWFDDVVFSYEI PPGVLDKERLKEIKKEYGNV AITLINPKPSLVKEAVQRFK QNYLIYVESSDLRVVRYSIE RGVDAVISPWANRKDQGIDH VLARMMNKRGVALGFSLRPL LHQNPYERANALKFMRKAWT LVNKYKVPRFISSSAKGKFQ VRGVKELISLGIAIGMEEVQ AKASLSFYPLGILERLK

Data sets:
Data typeCount
13C chemical shifts570
15N chemical shifts195
1H chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rpp301

Entities:

Entity 1, Rpp30 217 residues - Formula weight is not available

The 1st 3 residues are artifacted from a cleavage affinity tag. Residue 4 on is the sequence for Rpp30 from Pfu

1   GLYGLUPHEMETALAGLYGLYARGASNGLY
2   VALLYSPHEVALGLUMETASPILEARGSER
3   ARGGLUALATYRGLULEUALAGLUGLUTRP
4   PHEASPASPVALVALPHESERTYRGLUILE
5   PROPROGLYVALLEUASPLYSGLUARGLEU
6   LYSGLUILELYSLYSGLUTYRGLYASNVAL
7   ALAILETHRLEUILEASNPROLYSPROSER
8   LEUVALLYSGLUALAVALGLNARGPHELYS
9   GLNASNTYRLEUILETYRVALGLUSERSER
10   ASPLEUARGVALVALARGTYRSERILEGLU
11   ARGGLYVALASPALAVALILESERPROTRP
12   ALAASNARGLYSASPGLNGLYILEASPHIS
13   VALLEUALAARGMETMETASNLYSARGGLY
14   VALALALEUGLYPHESERLEUARGPROLEU
15   LEUHISGLNASNPROTYRGLUARGALAASN
16   ALALEULYSPHEMETARGLYSALATRPTHR
17   LEUVALASNLYSTYRLYSVALPROARGPHE
18   ILESERSERSERALALYSGLYLYSPHEGLN
19   VALARGGLYVALLYSGLULEUILESERLEU
20   GLYILEALAILEGLYMETGLUGLUVALGLN
21   ALALYSALASERLEUSERPHETYRPROLEU
22   GLYILELEUGLUARGLEULYS

Samples:

sample_1: Rpp30, [U-100% 13C; U-100% 15N], 500 uM; D2O 5%; H2O 95%; DSS 66.67 uM; TRIS 10 mM; Sodium Azide 0.02%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 17190
GB AAL82038 AFN04726
REF WP_011013054
SP Q8TZS0

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts