BMRB Entry 17144

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17144

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Title:
1H, 13C, and 15N backbone, side-chain, and heme chemical shift assignments for the oxidized form of the monoheme c-type cytochrome ApcA isolated from the acidophilic metal-reducing bacterium Acidiphilium cryptum
Deposition date:
2010-08-24
Original release date:
2010-10-04
Authors:
Cort, John; Swenson, Michael; Magnuson, Timothy
Citation:

Citation: Cort, John; Swenson, Michael; Magnuson, Timothy. "(1)H, (13)C, and (15)N backbone, side-chain, and heme chemical shift assignments for oxidized and reduced forms of the monoheme c-type cytochrome ApcA isolated from the acidophilic metal-reducing bacterium Acidiphilium cryptum."  Biomol. NMR Assignments 5, 89-92 (2011).
PubMed: 21197590

Assembly members:

Assembly members:
ApcA, polymer, 102 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 524   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acidiphilium cryptum

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Acidiphilium cryptum

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ApcA: ANVAHGKALFQAQCAACHSV SPGQNGIGPSLAGVYGAKAA ATPGFQFSPALKKSGIVWNA STLDKFLANPQADVPGTKMP YMGMANATDRADVVAYLQTL GK

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts108
1H chemical shifts663

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ApcA1
2heme2

Entities:

Entity 1, ApcA 102 residues - Formula weight is not available

An N-terminal 21 amino acid signal sequence is not present and presumably was cleaved by the host organism.

1   ALAASNVALALAHISGLYLYSALALEUPHE
2   GLNALAGLNCYSALAALACYSHISSERVAL
3   SERPROGLYGLNASNGLYILEGLYPROSER
4   LEUALAGLYVALTYRGLYALALYSALAALA
5   ALATHRPROGLYPHEGLNPHESERPROALA
6   LEULYSLYSSERGLYILEVALTRPASNALA
7   SERTHRLEUASPLYSPHELEUALAASNPRO
8   GLNALAASPVALPROGLYTHRLYSMETPRO
9   TYRMETGLYMETALAASNALATHRASPARG
10   ALAASPVALVALALATYRLEUGLNTHRLEU
11   GLYLYS

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Related Database Links:

NCBI YP_001235217.1
UNP A5G0B6
BMRB 17146
DBJ BAJ81692 GAN75771
GB ABQ31298 EGO93894 KDM67854
REF WP_007424422
AlphaFold A5G0B6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks