BMRB Entry 16754
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16754
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Citation: Stauffer, Melissa; Chazin, Walter. "Physical Interaction between Replication Protein A and Rad51 Promotes Exchange on Single-stranded DNA" J. Biol. Chem. 279, 25638-25645 (2004).
PubMed: 15056657
Assembly members:
RPA70A, polymer, 252 residues, Formula weight is not available
Rad51N, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
RPA70A: MSKVVPIASLTPYQSKWTIC
ARVTNKSQIRTWSNSRGEGK
LFSLELVDESGEIRATAFNE
QVDKFFPLIEVNKVYYFSKG
TLKIANKQFTAVKNDYEMTF
NNETSVMPCEDDHHLPTVQF
DFTGIDDLENKSKDSLVDII
GICKSYEDATKITVRSNNRE
VAKRNIYLMDTSGKVVTATL
WGEDADKFDGSRQPVLAIKG
ARVSDFGGRSLSVLSSSTII
ANPDIPEAYKLRGWFDAEGQ
ALDGVSISDLKS
Rad51N: MAMQMQLEANADTSVEEESF
GPQPISRLEQCGINANDVKK
LEEAGFHTVEAVAYAPKKEL
INIKGISEAKADKILAEAAK
LVPMGFTTATEFHQRRSEII
QITTGSKELDKLLQ
- binding_constants
| Data type | Count |
| binding constants | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RPA70A | 1 |
| 2 | Rad51N | 2 |
Entities:
Entity 1, RPA70A 252 residues - Formula weight is not available
| 1 | MET | SER | LYS | VAL | VAL | PRO | ILE | ALA | SER | LEU | ||||
| 2 | THR | PRO | TYR | GLN | SER | LYS | TRP | THR | ILE | CYS | ||||
| 3 | ALA | ARG | VAL | THR | ASN | LYS | SER | GLN | ILE | ARG | ||||
| 4 | THR | TRP | SER | ASN | SER | ARG | GLY | GLU | GLY | LYS | ||||
| 5 | LEU | PHE | SER | LEU | GLU | LEU | VAL | ASP | GLU | SER | ||||
| 6 | GLY | GLU | ILE | ARG | ALA | THR | ALA | PHE | ASN | GLU | ||||
| 7 | GLN | VAL | ASP | LYS | PHE | PHE | PRO | LEU | ILE | GLU | ||||
| 8 | VAL | ASN | LYS | VAL | TYR | TYR | PHE | SER | LYS | GLY | ||||
| 9 | THR | LEU | LYS | ILE | ALA | ASN | LYS | GLN | PHE | THR | ||||
| 10 | ALA | VAL | LYS | ASN | ASP | TYR | GLU | MET | THR | PHE | ||||
| 11 | ASN | ASN | GLU | THR | SER | VAL | MET | PRO | CYS | GLU | ||||
| 12 | ASP | ASP | HIS | HIS | LEU | PRO | THR | VAL | GLN | PHE | ||||
| 13 | ASP | PHE | THR | GLY | ILE | ASP | ASP | LEU | GLU | ASN | ||||
| 14 | LYS | SER | LYS | ASP | SER | LEU | VAL | ASP | ILE | ILE | ||||
| 15 | GLY | ILE | CYS | LYS | SER | TYR | GLU | ASP | ALA | THR | ||||
| 16 | LYS | ILE | THR | VAL | ARG | SER | ASN | ASN | ARG | GLU | ||||
| 17 | VAL | ALA | LYS | ARG | ASN | ILE | TYR | LEU | MET | ASP | ||||
| 18 | THR | SER | GLY | LYS | VAL | VAL | THR | ALA | THR | LEU | ||||
| 19 | TRP | GLY | GLU | ASP | ALA | ASP | LYS | PHE | ASP | GLY | ||||
| 20 | SER | ARG | GLN | PRO | VAL | LEU | ALA | ILE | LYS | GLY | ||||
| 21 | ALA | ARG | VAL | SER | ASP | PHE | GLY | GLY | ARG | SER | ||||
| 22 | LEU | SER | VAL | LEU | SER | SER | SER | THR | ILE | ILE | ||||
| 23 | ALA | ASN | PRO | ASP | ILE | PRO | GLU | ALA | TYR | LYS | ||||
| 24 | LEU | ARG | GLY | TRP | PHE | ASP | ALA | GLU | GLY | GLN | ||||
| 25 | ALA | LEU | ASP | GLY | VAL | SER | ILE | SER | ASP | LEU | ||||
| 26 | LYS | SER |
Entity 2, Rad51N 114 residues - Formula weight is not available
| 1 | MET | ALA | MET | GLN | MET | GLN | LEU | GLU | ALA | ASN | ||||
| 2 | ALA | ASP | THR | SER | VAL | GLU | GLU | GLU | SER | PHE | ||||
| 3 | GLY | PRO | GLN | PRO | ILE | SER | ARG | LEU | GLU | GLN | ||||
| 4 | CYS | GLY | ILE | ASN | ALA | ASN | ASP | VAL | LYS | LYS | ||||
| 5 | LEU | GLU | GLU | ALA | GLY | PHE | HIS | THR | VAL | GLU | ||||
| 6 | ALA | VAL | ALA | TYR | ALA | PRO | LYS | LYS | GLU | LEU | ||||
| 7 | ILE | ASN | ILE | LYS | GLY | ILE | SER | GLU | ALA | LYS | ||||
| 8 | ALA | ASP | LYS | ILE | LEU | ALA | GLU | ALA | ALA | LYS | ||||
| 9 | LEU | VAL | PRO | MET | GLY | PHE | THR | THR | ALA | THR | ||||
| 10 | GLU | PHE | HIS | GLN | ARG | ARG | SER | GLU | ILE | ILE | ||||
| 11 | GLN | ILE | THR | THR | GLY | SER | LYS | GLU | LEU | ASP | ||||
| 12 | LYS | LEU | LEU | GLN |
Samples:
sample_1: Rad51N, [U-15N], 0.3 – 0.8 mM; RPA70A, [U-15N], 0.3 – 0.8 mM; Tis 10 mM; Potassium Chloride 20 mM; BME 1 mM; sodium azide 1 uM; H2O 100%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX, Accelrys Software Inc. - processing
HADDOCK, Dominguez, C., Boelens, R., and Bonvin, A.M. 2003 - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92969 BAF82393 BAG10883 BAA02718 BAA02961 BAA02962 BAA03189 BAB27489 |
| EMBL | CAH92206 CAA69384 CAG38796 |
| GB | AAA36584 AAH18126 AAS94324 ADR83314 AIC58576 AAC28561 AAD49705 AAF61901 AAF69145 AAH27384 |
| REF | NP_001126290 NP_002936 XP_002747872 XP_002800290 XP_003277864 NP_001003043 NP_001039644 NP_001075493 NP_001102674 NP_001116653 |
| SP | P27694 Q5R7Q4 O77507 P70099 Q06609 Q08297 Q2KJ94 |
| BMRB | 11046 4328 |
| PRF | 2102359A |
| TPG | DAA25435 |
| AlphaFold | P27694 Q5R7Q4 O77507 P70099 Q06609 Q08297 Q2KJ94 |