BMRB Entry 11046
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11046
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems." J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448
Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
hsRad51, polymer, 339 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
DNA (5'-D(*DTP*DAP*DCP*DG)-3'): TACG
hsRad51: MAMQMQLEANADTSVEEESF
GPQPISRLEQCGINANDVKK
LEEAGFHTVEAVAYAPKKEL
INIKGISEAKADKILAEAAK
LVPMGFTTATEFHQRRSEII
QITTGSKELDKLLQGGIETG
SITEMFGEFRTGKTQICHTL
AVTCQLPIDRGGGEGKAMYI
DTEGTFRPERLLAVAERYGL
SGSDVLDNVAYARAFNTDHQ
TQLLYQASAMMVESRYALLI
VDSATALYRTDYSGRGELSA
RQMHLARFLRMLLRLADEFG
VAVVITNQVVAQVDGAAMFA
ADPKKPIGGNIIAHASTTRL
YLRKGRGETRICKIYDSPCL
PEAEAMFAINADGVGDAKD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 37 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 | 1 |
| 2 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 2 | 1 |
| 3 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 3 | 1 |
| 4 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 4 | 1 |
| 5 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 5 | 1 |
| 6 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 6 | 1 |
| 7 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 7 | 1 |
| 8 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 8 | 1 |
| 9 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 9 | 1 |
| 10 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 10 | 1 |
| 11 | hsRad51 | 2 |
Entities:
Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.
| 1 | DT | DA | DC | DG |
Entity 2, hsRad51 339 residues - Formula weight is not available
| 1 | MET | ALA | MET | GLN | MET | GLN | LEU | GLU | ALA | ASN | ||||
| 2 | ALA | ASP | THR | SER | VAL | GLU | GLU | GLU | SER | PHE | ||||
| 3 | GLY | PRO | GLN | PRO | ILE | SER | ARG | LEU | GLU | GLN | ||||
| 4 | CYS | GLY | ILE | ASN | ALA | ASN | ASP | VAL | LYS | LYS | ||||
| 5 | LEU | GLU | GLU | ALA | GLY | PHE | HIS | THR | VAL | GLU | ||||
| 6 | ALA | VAL | ALA | TYR | ALA | PRO | LYS | LYS | GLU | LEU | ||||
| 7 | ILE | ASN | ILE | LYS | GLY | ILE | SER | GLU | ALA | LYS | ||||
| 8 | ALA | ASP | LYS | ILE | LEU | ALA | GLU | ALA | ALA | LYS | ||||
| 9 | LEU | VAL | PRO | MET | GLY | PHE | THR | THR | ALA | THR | ||||
| 10 | GLU | PHE | HIS | GLN | ARG | ARG | SER | GLU | ILE | ILE | ||||
| 11 | GLN | ILE | THR | THR | GLY | SER | LYS | GLU | LEU | ASP | ||||
| 12 | LYS | LEU | LEU | GLN | GLY | GLY | ILE | GLU | THR | GLY | ||||
| 13 | SER | ILE | THR | GLU | MET | PHE | GLY | GLU | PHE | ARG | ||||
| 14 | THR | GLY | LYS | THR | GLN | ILE | CYS | HIS | THR | LEU | ||||
| 15 | ALA | VAL | THR | CYS | GLN | LEU | PRO | ILE | ASP | ARG | ||||
| 16 | GLY | GLY | GLY | GLU | GLY | LYS | ALA | MET | TYR | ILE | ||||
| 17 | ASP | THR | GLU | GLY | THR | PHE | ARG | PRO | GLU | ARG | ||||
| 18 | LEU | LEU | ALA | VAL | ALA | GLU | ARG | TYR | GLY | LEU | ||||
| 19 | SER | GLY | SER | ASP | VAL | LEU | ASP | ASN | VAL | ALA | ||||
| 20 | TYR | ALA | ARG | ALA | PHE | ASN | THR | ASP | HIS | GLN | ||||
| 21 | THR | GLN | LEU | LEU | TYR | GLN | ALA | SER | ALA | MET | ||||
| 22 | MET | VAL | GLU | SER | ARG | TYR | ALA | LEU | LEU | ILE | ||||
| 23 | VAL | ASP | SER | ALA | THR | ALA | LEU | TYR | ARG | THR | ||||
| 24 | ASP | TYR | SER | GLY | ARG | GLY | GLU | LEU | SER | ALA | ||||
| 25 | ARG | GLN | MET | HIS | LEU | ALA | ARG | PHE | LEU | ARG | ||||
| 26 | MET | LEU | LEU | ARG | LEU | ALA | ASP | GLU | PHE | GLY | ||||
| 27 | VAL | ALA | VAL | VAL | ILE | THR | ASN | GLN | VAL | VAL | ||||
| 28 | ALA | GLN | VAL | ASP | GLY | ALA | ALA | MET | PHE | ALA | ||||
| 29 | ALA | ASP | PRO | LYS | LYS | PRO | ILE | GLY | GLY | ASN | ||||
| 30 | ILE | ILE | ALA | HIS | ALA | SER | THR | THR | ARG | LEU | ||||
| 31 | TYR | LEU | ARG | LYS | GLY | ARG | GLY | GLU | THR | ARG | ||||
| 32 | ILE | CYS | LYS | ILE | TYR | ASP | SER | PRO | CYS | LEU | ||||
| 33 | PRO | GLU | ALA | GLU | ALA | MET | PHE | ALA | ILE | ASN | ||||
| 34 | ALA | ASP | GLY | VAL | GLY | ASP | ALA | LYS | ASP |
Samples:
sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 0.5 mM; hsRad51 0.05 mM; AMP-PNP 10 mM; MgCl2 5 mM; NaCl 100 mM; D2O 100%
sample_conditions_1: ionic strength: 100 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| transferred NOESY | sample_1 | isotropic | sample_conditions_1 |
| DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| TOCSY | sample_1 | isotropic | sample_conditions_1 |
| ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| DBJ | BAA02718 BAA02961 BAA02962 BAA03189 BAB27489 |
| EMBL | CAA69384 CAG38796 |
| GB | AAC28561 AAD49705 AAF69145 AAH27384 AAI05459 |
| PRF | 2102359A |
| REF | NP_001003043 NP_001039644 NP_001075493 NP_001102674 NP_001116653 |
| SP | O77507 P70099 Q06609 Q08297 Q2KJ94 |
| TPG | DAA25435 |
| AlphaFold | O77507 P70099 Q06609 Q08297 Q2KJ94 |