data_16754 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 16754 _Entry.Title ; Physical Interaction between Replication Protein A and Rad51 Promotes Exchange on Single-stranded DNA ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-03-03 _Entry.Accession_date 2010-03-03 _Entry.Last_release_date 2010-03-24 _Entry.Original_release_date 2010-03-24 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Melissa Stauffer . E. . 16754 2 Walter Chazin . J. . 16754 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 16754 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 1 16754 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-03-24 2010-03-03 original author . 16754 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 16754 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 15056657 _Citation.Full_citation . _Citation.Title 'Physical Interaction between Replication Protein A and Rad51 Promotes Exchange on Single-stranded DNA' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Biol. Chem.' _Citation.Journal_name_full . _Citation.Journal_volume 279 _Citation.Journal_issue 24 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 25638 _Citation.Page_last 25645 _Citation.Year 2004 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Melissa Stauffer . E. . 16754 1 2 Walter Chazin . J. . 16754 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 16754 _Assembly.ID 1 _Assembly.Name 'RPA70A Rad51N complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 RPA70A 1 $RPA70A A . yes native no no . . . 16754 1 2 Rad51N 2 $Rad51N B . no native no no . . . 16754 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_RPA70A _Entity.Sf_category entity _Entity.Sf_framecode RPA70A _Entity.Entry_ID 16754 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name RPA70A _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MSKVVPIASLTPYQSKWTIC ARVTNKSQIRTWSNSRGEGK LFSLELVDESGEIRATAFNE QVDKFFPLIEVNKVYYFSKG TLKIANKQFTAVKNDYEMTF NNETSVMPCEDDHHLPTVQF DFTGIDDLENKSKDSLVDII GICKSYEDATKITVRSNNRE VAKRNIYLMDTSGKVVTATL WGEDADKFDGSRQPVLAIKG ARVSDFGGRSLSVLSSSTII ANPDIPEAYKLRGWFDAEGQ ALDGVSISDLKS ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 252 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no PDB 1FGU . "Ssdna-Binding Domain Of The Large Subunit Of Replication Protein A" . . . . . 100.00 252 100.00 100.00 0.00e+00 . . . . 16754 1 2 no PDB 1JMC . "Single Stranded Dna-Binding Domain Of Human Replication Protein A Bound To Single Stranded Dna, Rpa70 Subunit, Residues 183-420" . . . . . 95.63 246 100.00 100.00 5.33e-177 . . . . 16754 1 3 no DBJ BAD92969 . "replication protein A1, 70kDa variant [Homo sapiens]" . . . . . 99.60 630 99.60 99.60 1.41e-178 . . . . 16754 1 4 no DBJ BAF82393 . "unnamed protein product [Homo sapiens]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 5 no DBJ BAG10883 . "replication protein A 70 kDa DNA-binding subunit [synthetic construct]" . . . . . 99.60 616 99.60 99.60 9.11e-179 . . . . 16754 1 6 no EMBL CAH92206 . "hypothetical protein [Pongo abelii]" . . . . . 99.60 616 99.60 99.60 4.36e-178 . . . . 16754 1 7 no GB AAA36584 . "replication protein A, 70-kDa subunit [Homo sapiens]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 8 no GB AAH18126 . "Replication protein A1, 70kDa [Homo sapiens]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 9 no GB AAS94324 . "replication protein A1, 70kDa [Homo sapiens]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 10 no GB ADR83314 . "replication protein A1, 70kDa [synthetic construct]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 11 no GB AIC58576 . "RPA1, partial [synthetic construct]" . . . . . 99.60 616 100.00 100.00 2.55e-179 . . . . 16754 1 12 no REF NP_001126290 . "replication protein A 70 kDa DNA-binding subunit [Pongo abelii]" . . . . . 99.60 616 99.60 99.60 4.36e-178 . . . . 16754 1 13 no REF NP_002936 . "replication protein A 70 kDa DNA-binding subunit [Homo sapiens]" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 14 no REF XP_002747872 . "PREDICTED: replication protein A 70 kDa DNA-binding subunit isoform X1 [Callithrix jacchus]" . . . . . 99.60 616 97.61 99.20 1.18e-175 . . . . 16754 1 15 no REF XP_002800290 . "PREDICTED: replication protein A 70 kDa DNA-binding subunit-like, partial [Macaca mulatta]" . . . . . 99.60 532 99.20 99.60 2.15e-178 . . . . 16754 1 16 no REF XP_003277864 . "PREDICTED: replication protein A 70 kDa DNA-binding subunit [Nomascus leucogenys]" . . . . . 99.60 616 99.60 99.60 7.41e-179 . . . . 16754 1 17 no SP P27694 . "RecName: Full=Replication protein A 70 kDa DNA-binding subunit; Short=RP-A p70; AltName: Full=Replication factor A protein 1; S" . . . . . 99.60 616 100.00 100.00 1.78e-179 . . . . 16754 1 18 no SP Q5R7Q4 . "RecName: Full=Replication protein A 70 kDa DNA-binding subunit; Short=RP-A p70; AltName: Full=Replication factor A protein 1; S" . . . . . 99.60 616 99.60 99.60 4.36e-178 . . . . 16754 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 16754 1 2 . SER . 16754 1 3 . LYS . 16754 1 4 . VAL . 16754 1 5 . VAL . 16754 1 6 . PRO . 16754 1 7 . ILE . 16754 1 8 . ALA . 16754 1 9 . SER . 16754 1 10 . LEU . 16754 1 11 . THR . 16754 1 12 . PRO . 16754 1 13 . TYR . 16754 1 14 . GLN . 16754 1 15 . SER . 16754 1 16 . LYS . 16754 1 17 . TRP . 16754 1 18 . THR . 16754 1 19 . ILE . 16754 1 20 . CYS . 16754 1 21 . ALA . 16754 1 22 . ARG . 16754 1 23 . VAL . 16754 1 24 . THR . 16754 1 25 . ASN . 16754 1 26 . LYS . 16754 1 27 . SER . 16754 1 28 . GLN . 16754 1 29 . ILE . 16754 1 30 . ARG . 16754 1 31 . THR . 16754 1 32 . TRP . 16754 1 33 . SER . 16754 1 34 . ASN . 16754 1 35 . SER . 16754 1 36 . ARG . 16754 1 37 . GLY . 16754 1 38 . GLU . 16754 1 39 . GLY . 16754 1 40 . LYS . 16754 1 41 . LEU . 16754 1 42 . PHE . 16754 1 43 . SER . 16754 1 44 . LEU . 16754 1 45 . GLU . 16754 1 46 . LEU . 16754 1 47 . VAL . 16754 1 48 . ASP . 16754 1 49 . GLU . 16754 1 50 . SER . 16754 1 51 . GLY . 16754 1 52 . GLU . 16754 1 53 . ILE . 16754 1 54 . ARG . 16754 1 55 . ALA . 16754 1 56 . THR . 16754 1 57 . ALA . 16754 1 58 . PHE . 16754 1 59 . ASN . 16754 1 60 . GLU . 16754 1 61 . GLN . 16754 1 62 . VAL . 16754 1 63 . ASP . 16754 1 64 . LYS . 16754 1 65 . PHE . 16754 1 66 . PHE . 16754 1 67 . PRO . 16754 1 68 . LEU . 16754 1 69 . ILE . 16754 1 70 . GLU . 16754 1 71 . VAL . 16754 1 72 . ASN . 16754 1 73 . LYS . 16754 1 74 . VAL . 16754 1 75 . TYR . 16754 1 76 . TYR . 16754 1 77 . PHE . 16754 1 78 . SER . 16754 1 79 . LYS . 16754 1 80 . GLY . 16754 1 81 . THR . 16754 1 82 . LEU . 16754 1 83 . LYS . 16754 1 84 . ILE . 16754 1 85 . ALA . 16754 1 86 . ASN . 16754 1 87 . LYS . 16754 1 88 . GLN . 16754 1 89 . PHE . 16754 1 90 . THR . 16754 1 91 . ALA . 16754 1 92 . VAL . 16754 1 93 . LYS . 16754 1 94 . ASN . 16754 1 95 . ASP . 16754 1 96 . TYR . 16754 1 97 . GLU . 16754 1 98 . MET . 16754 1 99 . THR . 16754 1 100 . PHE . 16754 1 101 . ASN . 16754 1 102 . ASN . 16754 1 103 . GLU . 16754 1 104 . THR . 16754 1 105 . SER . 16754 1 106 . VAL . 16754 1 107 . MET . 16754 1 108 . PRO . 16754 1 109 . CYS . 16754 1 110 . GLU . 16754 1 111 . ASP . 16754 1 112 . ASP . 16754 1 113 . HIS . 16754 1 114 . HIS . 16754 1 115 . LEU . 16754 1 116 . PRO . 16754 1 117 . THR . 16754 1 118 . VAL . 16754 1 119 . GLN . 16754 1 120 . PHE . 16754 1 121 . ASP . 16754 1 122 . PHE . 16754 1 123 . THR . 16754 1 124 . GLY . 16754 1 125 . ILE . 16754 1 126 . ASP . 16754 1 127 . ASP . 16754 1 128 . LEU . 16754 1 129 . GLU . 16754 1 130 . ASN . 16754 1 131 . LYS . 16754 1 132 . SER . 16754 1 133 . LYS . 16754 1 134 . ASP . 16754 1 135 . SER . 16754 1 136 . LEU . 16754 1 137 . VAL . 16754 1 138 . ASP . 16754 1 139 . ILE . 16754 1 140 . ILE . 16754 1 141 . GLY . 16754 1 142 . ILE . 16754 1 143 . CYS . 16754 1 144 . LYS . 16754 1 145 . SER . 16754 1 146 . TYR . 16754 1 147 . GLU . 16754 1 148 . ASP . 16754 1 149 . ALA . 16754 1 150 . THR . 16754 1 151 . LYS . 16754 1 152 . ILE . 16754 1 153 . THR . 16754 1 154 . VAL . 16754 1 155 . ARG . 16754 1 156 . SER . 16754 1 157 . ASN . 16754 1 158 . ASN . 16754 1 159 . ARG . 16754 1 160 . GLU . 16754 1 161 . VAL . 16754 1 162 . ALA . 16754 1 163 . LYS . 16754 1 164 . ARG . 16754 1 165 . ASN . 16754 1 166 . ILE . 16754 1 167 . TYR . 16754 1 168 . LEU . 16754 1 169 . MET . 16754 1 170 . ASP . 16754 1 171 . THR . 16754 1 172 . SER . 16754 1 173 . GLY . 16754 1 174 . LYS . 16754 1 175 . VAL . 16754 1 176 . VAL . 16754 1 177 . THR . 16754 1 178 . ALA . 16754 1 179 . THR . 16754 1 180 . LEU . 16754 1 181 . TRP . 16754 1 182 . GLY . 16754 1 183 . GLU . 16754 1 184 . ASP . 16754 1 185 . ALA . 16754 1 186 . ASP . 16754 1 187 . LYS . 16754 1 188 . PHE . 16754 1 189 . ASP . 16754 1 190 . GLY . 16754 1 191 . SER . 16754 1 192 . ARG . 16754 1 193 . GLN . 16754 1 194 . PRO . 16754 1 195 . VAL . 16754 1 196 . LEU . 16754 1 197 . ALA . 16754 1 198 . ILE . 16754 1 199 . LYS . 16754 1 200 . GLY . 16754 1 201 . ALA . 16754 1 202 . ARG . 16754 1 203 . VAL . 16754 1 204 . SER . 16754 1 205 . ASP . 16754 1 206 . PHE . 16754 1 207 . GLY . 16754 1 208 . GLY . 16754 1 209 . ARG . 16754 1 210 . SER . 16754 1 211 . LEU . 16754 1 212 . SER . 16754 1 213 . VAL . 16754 1 214 . LEU . 16754 1 215 . SER . 16754 1 216 . SER . 16754 1 217 . SER . 16754 1 218 . THR . 16754 1 219 . ILE . 16754 1 220 . ILE . 16754 1 221 . ALA . 16754 1 222 . ASN . 16754 1 223 . PRO . 16754 1 224 . ASP . 16754 1 225 . ILE . 16754 1 226 . PRO . 16754 1 227 . GLU . 16754 1 228 . ALA . 16754 1 229 . TYR . 16754 1 230 . LYS . 16754 1 231 . LEU . 16754 1 232 . ARG . 16754 1 233 . GLY . 16754 1 234 . TRP . 16754 1 235 . PHE . 16754 1 236 . ASP . 16754 1 237 . ALA . 16754 1 238 . GLU . 16754 1 239 . GLY . 16754 1 240 . GLN . 16754 1 241 . ALA . 16754 1 242 . LEU . 16754 1 243 . ASP . 16754 1 244 . GLY . 16754 1 245 . VAL . 16754 1 246 . SER . 16754 1 247 . ILE . 16754 1 248 . SER . 16754 1 249 . ASP . 16754 1 250 . LEU . 16754 1 251 . LYS . 16754 1 252 . SER . 16754 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 16754 1 . SER 2 2 16754 1 . LYS 3 3 16754 1 . VAL 4 4 16754 1 . VAL 5 5 16754 1 . PRO 6 6 16754 1 . ILE 7 7 16754 1 . ALA 8 8 16754 1 . SER 9 9 16754 1 . LEU 10 10 16754 1 . THR 11 11 16754 1 . PRO 12 12 16754 1 . TYR 13 13 16754 1 . GLN 14 14 16754 1 . SER 15 15 16754 1 . LYS 16 16 16754 1 . TRP 17 17 16754 1 . THR 18 18 16754 1 . ILE 19 19 16754 1 . CYS 20 20 16754 1 . ALA 21 21 16754 1 . ARG 22 22 16754 1 . VAL 23 23 16754 1 . THR 24 24 16754 1 . ASN 25 25 16754 1 . LYS 26 26 16754 1 . SER 27 27 16754 1 . GLN 28 28 16754 1 . ILE 29 29 16754 1 . ARG 30 30 16754 1 . THR 31 31 16754 1 . TRP 32 32 16754 1 . SER 33 33 16754 1 . ASN 34 34 16754 1 . SER 35 35 16754 1 . ARG 36 36 16754 1 . GLY 37 37 16754 1 . GLU 38 38 16754 1 . GLY 39 39 16754 1 . LYS 40 40 16754 1 . LEU 41 41 16754 1 . PHE 42 42 16754 1 . SER 43 43 16754 1 . LEU 44 44 16754 1 . GLU 45 45 16754 1 . LEU 46 46 16754 1 . VAL 47 47 16754 1 . ASP 48 48 16754 1 . GLU 49 49 16754 1 . SER 50 50 16754 1 . GLY 51 51 16754 1 . GLU 52 52 16754 1 . ILE 53 53 16754 1 . ARG 54 54 16754 1 . ALA 55 55 16754 1 . THR 56 56 16754 1 . ALA 57 57 16754 1 . PHE 58 58 16754 1 . ASN 59 59 16754 1 . GLU 60 60 16754 1 . GLN 61 61 16754 1 . VAL 62 62 16754 1 . ASP 63 63 16754 1 . LYS 64 64 16754 1 . PHE 65 65 16754 1 . PHE 66 66 16754 1 . PRO 67 67 16754 1 . LEU 68 68 16754 1 . ILE 69 69 16754 1 . GLU 70 70 16754 1 . VAL 71 71 16754 1 . ASN 72 72 16754 1 . LYS 73 73 16754 1 . VAL 74 74 16754 1 . TYR 75 75 16754 1 . TYR 76 76 16754 1 . PHE 77 77 16754 1 . SER 78 78 16754 1 . LYS 79 79 16754 1 . GLY 80 80 16754 1 . THR 81 81 16754 1 . LEU 82 82 16754 1 . LYS 83 83 16754 1 . ILE 84 84 16754 1 . ALA 85 85 16754 1 . ASN 86 86 16754 1 . LYS 87 87 16754 1 . GLN 88 88 16754 1 . PHE 89 89 16754 1 . THR 90 90 16754 1 . ALA 91 91 16754 1 . VAL 92 92 16754 1 . LYS 93 93 16754 1 . ASN 94 94 16754 1 . ASP 95 95 16754 1 . TYR 96 96 16754 1 . GLU 97 97 16754 1 . MET 98 98 16754 1 . THR 99 99 16754 1 . PHE 100 100 16754 1 . ASN 101 101 16754 1 . ASN 102 102 16754 1 . GLU 103 103 16754 1 . THR 104 104 16754 1 . SER 105 105 16754 1 . VAL 106 106 16754 1 . MET 107 107 16754 1 . PRO 108 108 16754 1 . CYS 109 109 16754 1 . GLU 110 110 16754 1 . ASP 111 111 16754 1 . ASP 112 112 16754 1 . HIS 113 113 16754 1 . HIS 114 114 16754 1 . LEU 115 115 16754 1 . PRO 116 116 16754 1 . THR 117 117 16754 1 . VAL 118 118 16754 1 . GLN 119 119 16754 1 . PHE 120 120 16754 1 . ASP 121 121 16754 1 . PHE 122 122 16754 1 . THR 123 123 16754 1 . GLY 124 124 16754 1 . ILE 125 125 16754 1 . ASP 126 126 16754 1 . ASP 127 127 16754 1 . LEU 128 128 16754 1 . GLU 129 129 16754 1 . ASN 130 130 16754 1 . LYS 131 131 16754 1 . SER 132 132 16754 1 . LYS 133 133 16754 1 . ASP 134 134 16754 1 . SER 135 135 16754 1 . LEU 136 136 16754 1 . VAL 137 137 16754 1 . ASP 138 138 16754 1 . ILE 139 139 16754 1 . ILE 140 140 16754 1 . GLY 141 141 16754 1 . ILE 142 142 16754 1 . CYS 143 143 16754 1 . LYS 144 144 16754 1 . SER 145 145 16754 1 . TYR 146 146 16754 1 . GLU 147 147 16754 1 . ASP 148 148 16754 1 . ALA 149 149 16754 1 . THR 150 150 16754 1 . LYS 151 151 16754 1 . ILE 152 152 16754 1 . THR 153 153 16754 1 . VAL 154 154 16754 1 . ARG 155 155 16754 1 . SER 156 156 16754 1 . ASN 157 157 16754 1 . ASN 158 158 16754 1 . ARG 159 159 16754 1 . GLU 160 160 16754 1 . VAL 161 161 16754 1 . ALA 162 162 16754 1 . LYS 163 163 16754 1 . ARG 164 164 16754 1 . ASN 165 165 16754 1 . ILE 166 166 16754 1 . TYR 167 167 16754 1 . LEU 168 168 16754 1 . MET 169 169 16754 1 . ASP 170 170 16754 1 . THR 171 171 16754 1 . SER 172 172 16754 1 . GLY 173 173 16754 1 . LYS 174 174 16754 1 . VAL 175 175 16754 1 . VAL 176 176 16754 1 . THR 177 177 16754 1 . ALA 178 178 16754 1 . THR 179 179 16754 1 . LEU 180 180 16754 1 . TRP 181 181 16754 1 . GLY 182 182 16754 1 . GLU 183 183 16754 1 . ASP 184 184 16754 1 . ALA 185 185 16754 1 . ASP 186 186 16754 1 . LYS 187 187 16754 1 . PHE 188 188 16754 1 . ASP 189 189 16754 1 . GLY 190 190 16754 1 . SER 191 191 16754 1 . ARG 192 192 16754 1 . GLN 193 193 16754 1 . PRO 194 194 16754 1 . VAL 195 195 16754 1 . LEU 196 196 16754 1 . ALA 197 197 16754 1 . ILE 198 198 16754 1 . LYS 199 199 16754 1 . GLY 200 200 16754 1 . ALA 201 201 16754 1 . ARG 202 202 16754 1 . VAL 203 203 16754 1 . SER 204 204 16754 1 . ASP 205 205 16754 1 . PHE 206 206 16754 1 . GLY 207 207 16754 1 . GLY 208 208 16754 1 . ARG 209 209 16754 1 . SER 210 210 16754 1 . LEU 211 211 16754 1 . SER 212 212 16754 1 . VAL 213 213 16754 1 . LEU 214 214 16754 1 . SER 215 215 16754 1 . SER 216 216 16754 1 . SER 217 217 16754 1 . THR 218 218 16754 1 . ILE 219 219 16754 1 . ILE 220 220 16754 1 . ALA 221 221 16754 1 . ASN 222 222 16754 1 . PRO 223 223 16754 1 . ASP 224 224 16754 1 . ILE 225 225 16754 1 . PRO 226 226 16754 1 . GLU 227 227 16754 1 . ALA 228 228 16754 1 . TYR 229 229 16754 1 . LYS 230 230 16754 1 . LEU 231 231 16754 1 . ARG 232 232 16754 1 . GLY 233 233 16754 1 . TRP 234 234 16754 1 . PHE 235 235 16754 1 . ASP 236 236 16754 1 . ALA 237 237 16754 1 . GLU 238 238 16754 1 . GLY 239 239 16754 1 . GLN 240 240 16754 1 . ALA 241 241 16754 1 . LEU 242 242 16754 1 . ASP 243 243 16754 1 . GLY 244 244 16754 1 . VAL 245 245 16754 1 . SER 246 246 16754 1 . ILE 247 247 16754 1 . SER 248 248 16754 1 . ASP 249 249 16754 1 . LEU 250 250 16754 1 . LYS 251 251 16754 1 . SER 252 252 16754 1 stop_ save_ save_Rad51N _Entity.Sf_category entity _Entity.Sf_framecode Rad51N _Entity.Entry_ID 16754 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name Rad51N _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MAMQMQLEANADTSVEEESF GPQPISRLEQCGINANDVKK LEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAK LVPMGFTTATEFHQRRSEII QITTGSKELDKLLQ ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 114 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 11046 . hsRad51 . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 2 no BMRB 4328 . HsRad51(1-114) . . . . . 100.00 114 100.00 100.00 4.24e-76 . . . . 16754 2 3 no PDB 1B22 . "Rad51 (N-Terminal Domain)" . . . . . 100.00 114 100.00 100.00 4.24e-76 . . . . 16754 2 4 no DBJ BAA02718 . "Rad51 protein [Mus musculus]" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 5 no DBJ BAA02961 . "MmRad51 [Mus musculus]" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 6 no DBJ BAA02962 . "HsRad51 [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.34e-73 . . . . 16754 2 7 no DBJ BAA03189 . "RAD51 [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 8 no DBJ BAB27489 . "unnamed protein product [Mus musculus]" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 9 no EMBL CAA69384 . "rad51 [Cricetulus griseus]" . . . . . 100.00 339 99.12 100.00 2.36e-72 . . . . 16754 2 10 no EMBL CAG38796 . "RAD51 [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 11 no GB AAC28561 . "Rad51 [Oryctolagus cuniculus]" . . . . . 100.00 339 97.37 98.25 5.24e-71 . . . . 16754 2 12 no GB AAD49705 . "Rad51 [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 13 no GB AAF61901 . "RAD51 [Homo sapiens]" . . . . . 65.79 75 100.00 100.00 8.76e-46 . . . . 16754 2 14 no GB AAF69145 . "RAD51 [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 15 no GB AAH27384 . "RAD51 homolog (S. cerevisiae) [Mus musculus]" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 16 no PRF 2102359A . "RAD51-like protein" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 17 no REF NP_001003043 . "DNA repair protein RAD51 homolog 1 [Canis lupus familiaris]" . . . . . 100.00 339 98.25 99.12 1.12e-71 . . . . 16754 2 18 no REF NP_001039644 . "DNA repair protein RAD51 homolog 1 [Bos taurus]" . . . . . 100.00 339 99.12 99.12 1.60e-72 . . . . 16754 2 19 no REF NP_001075493 . "DNA repair protein RAD51 homolog 1 [Oryctolagus cuniculus]" . . . . . 100.00 339 97.37 98.25 5.24e-71 . . . . 16754 2 20 no REF NP_001102674 . "DNA repair protein RAD51 homolog 1 [Rattus norvegicus]" . . . . . 100.00 339 99.12 100.00 1.07e-72 . . . . 16754 2 21 no REF NP_001116653 . "DNA repair protein RAD51 homolog 1 [Sus scrofa]" . . . . . 100.00 339 97.37 99.12 2.31e-71 . . . . 16754 2 22 no SP O77507 . "RecName: Full=DNA repair protein RAD51 homolog 1 [Oryctolagus cuniculus]" . . . . . 100.00 339 97.37 98.25 5.24e-71 . . . . 16754 2 23 no SP P70099 . "RecName: Full=DNA repair protein RAD51 homolog 1 [Cricetulus griseus]" . . . . . 100.00 339 99.12 100.00 2.36e-72 . . . . 16754 2 24 no SP Q06609 . "RecName: Full=DNA repair protein RAD51 homolog 1; Short=HsRAD51; Short=hRAD51; AltName: Full=RAD51 homolog A [Homo sapiens]" . . . . . 100.00 339 100.00 100.00 3.38e-73 . . . . 16754 2 25 no SP Q08297 . "RecName: Full=DNA repair protein RAD51 homolog 1; AltName: Full=RAD51 homolog A [Mus musculus]" . . . . . 100.00 339 97.37 99.12 2.41e-71 . . . . 16754 2 26 no SP Q2KJ94 . "RecName: Full=DNA repair protein RAD51 homolog 1 [Bos taurus]" . . . . . 100.00 339 99.12 99.12 1.60e-72 . . . . 16754 2 27 no TPG DAA25435 . "TPA: DNA repair protein RAD51 homolog 1 [Bos taurus]" . . . . . 100.00 339 99.12 99.12 1.60e-72 . . . . 16754 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 16754 2 2 . ALA . 16754 2 3 . MET . 16754 2 4 . GLN . 16754 2 5 . MET . 16754 2 6 . GLN . 16754 2 7 . LEU . 16754 2 8 . GLU . 16754 2 9 . ALA . 16754 2 10 . ASN . 16754 2 11 . ALA . 16754 2 12 . ASP . 16754 2 13 . THR . 16754 2 14 . SER . 16754 2 15 . VAL . 16754 2 16 . GLU . 16754 2 17 . GLU . 16754 2 18 . GLU . 16754 2 19 . SER . 16754 2 20 . PHE . 16754 2 21 . GLY . 16754 2 22 . PRO . 16754 2 23 . GLN . 16754 2 24 . PRO . 16754 2 25 . ILE . 16754 2 26 . SER . 16754 2 27 . ARG . 16754 2 28 . LEU . 16754 2 29 . GLU . 16754 2 30 . GLN . 16754 2 31 . CYS . 16754 2 32 . GLY . 16754 2 33 . ILE . 16754 2 34 . ASN . 16754 2 35 . ALA . 16754 2 36 . ASN . 16754 2 37 . ASP . 16754 2 38 . VAL . 16754 2 39 . LYS . 16754 2 40 . LYS . 16754 2 41 . LEU . 16754 2 42 . GLU . 16754 2 43 . GLU . 16754 2 44 . ALA . 16754 2 45 . GLY . 16754 2 46 . PHE . 16754 2 47 . HIS . 16754 2 48 . THR . 16754 2 49 . VAL . 16754 2 50 . GLU . 16754 2 51 . ALA . 16754 2 52 . VAL . 16754 2 53 . ALA . 16754 2 54 . TYR . 16754 2 55 . ALA . 16754 2 56 . PRO . 16754 2 57 . LYS . 16754 2 58 . LYS . 16754 2 59 . GLU . 16754 2 60 . LEU . 16754 2 61 . ILE . 16754 2 62 . ASN . 16754 2 63 . ILE . 16754 2 64 . LYS . 16754 2 65 . GLY . 16754 2 66 . ILE . 16754 2 67 . SER . 16754 2 68 . GLU . 16754 2 69 . ALA . 16754 2 70 . LYS . 16754 2 71 . ALA . 16754 2 72 . ASP . 16754 2 73 . LYS . 16754 2 74 . ILE . 16754 2 75 . LEU . 16754 2 76 . ALA . 16754 2 77 . GLU . 16754 2 78 . ALA . 16754 2 79 . ALA . 16754 2 80 . LYS . 16754 2 81 . LEU . 16754 2 82 . VAL . 16754 2 83 . PRO . 16754 2 84 . MET . 16754 2 85 . GLY . 16754 2 86 . PHE . 16754 2 87 . THR . 16754 2 88 . THR . 16754 2 89 . ALA . 16754 2 90 . THR . 16754 2 91 . GLU . 16754 2 92 . PHE . 16754 2 93 . HIS . 16754 2 94 . GLN . 16754 2 95 . ARG . 16754 2 96 . ARG . 16754 2 97 . SER . 16754 2 98 . GLU . 16754 2 99 . ILE . 16754 2 100 . ILE . 16754 2 101 . GLN . 16754 2 102 . ILE . 16754 2 103 . THR . 16754 2 104 . THR . 16754 2 105 . GLY . 16754 2 106 . SER . 16754 2 107 . LYS . 16754 2 108 . GLU . 16754 2 109 . LEU . 16754 2 110 . ASP . 16754 2 111 . LYS . 16754 2 112 . LEU . 16754 2 113 . LEU . 16754 2 114 . GLN . 16754 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 16754 2 . ALA 2 2 16754 2 . MET 3 3 16754 2 . GLN 4 4 16754 2 . MET 5 5 16754 2 . GLN 6 6 16754 2 . LEU 7 7 16754 2 . GLU 8 8 16754 2 . ALA 9 9 16754 2 . ASN 10 10 16754 2 . ALA 11 11 16754 2 . ASP 12 12 16754 2 . THR 13 13 16754 2 . SER 14 14 16754 2 . VAL 15 15 16754 2 . GLU 16 16 16754 2 . GLU 17 17 16754 2 . GLU 18 18 16754 2 . SER 19 19 16754 2 . PHE 20 20 16754 2 . GLY 21 21 16754 2 . PRO 22 22 16754 2 . GLN 23 23 16754 2 . PRO 24 24 16754 2 . ILE 25 25 16754 2 . SER 26 26 16754 2 . ARG 27 27 16754 2 . LEU 28 28 16754 2 . GLU 29 29 16754 2 . GLN 30 30 16754 2 . CYS 31 31 16754 2 . GLY 32 32 16754 2 . ILE 33 33 16754 2 . ASN 34 34 16754 2 . ALA 35 35 16754 2 . ASN 36 36 16754 2 . ASP 37 37 16754 2 . VAL 38 38 16754 2 . LYS 39 39 16754 2 . LYS 40 40 16754 2 . LEU 41 41 16754 2 . GLU 42 42 16754 2 . GLU 43 43 16754 2 . ALA 44 44 16754 2 . GLY 45 45 16754 2 . PHE 46 46 16754 2 . HIS 47 47 16754 2 . THR 48 48 16754 2 . VAL 49 49 16754 2 . GLU 50 50 16754 2 . ALA 51 51 16754 2 . VAL 52 52 16754 2 . ALA 53 53 16754 2 . TYR 54 54 16754 2 . ALA 55 55 16754 2 . PRO 56 56 16754 2 . LYS 57 57 16754 2 . LYS 58 58 16754 2 . GLU 59 59 16754 2 . LEU 60 60 16754 2 . ILE 61 61 16754 2 . ASN 62 62 16754 2 . ILE 63 63 16754 2 . LYS 64 64 16754 2 . GLY 65 65 16754 2 . ILE 66 66 16754 2 . SER 67 67 16754 2 . GLU 68 68 16754 2 . ALA 69 69 16754 2 . LYS 70 70 16754 2 . ALA 71 71 16754 2 . ASP 72 72 16754 2 . LYS 73 73 16754 2 . ILE 74 74 16754 2 . LEU 75 75 16754 2 . ALA 76 76 16754 2 . GLU 77 77 16754 2 . ALA 78 78 16754 2 . ALA 79 79 16754 2 . LYS 80 80 16754 2 . LEU 81 81 16754 2 . VAL 82 82 16754 2 . PRO 83 83 16754 2 . MET 84 84 16754 2 . GLY 85 85 16754 2 . PHE 86 86 16754 2 . THR 87 87 16754 2 . THR 88 88 16754 2 . ALA 89 89 16754 2 . THR 90 90 16754 2 . GLU 91 91 16754 2 . PHE 92 92 16754 2 . HIS 93 93 16754 2 . GLN 94 94 16754 2 . ARG 95 95 16754 2 . ARG 96 96 16754 2 . SER 97 97 16754 2 . GLU 98 98 16754 2 . ILE 99 99 16754 2 . ILE 100 100 16754 2 . GLN 101 101 16754 2 . ILE 102 102 16754 2 . THR 103 103 16754 2 . THR 104 104 16754 2 . GLY 105 105 16754 2 . SER 106 106 16754 2 . LYS 107 107 16754 2 . GLU 108 108 16754 2 . LEU 109 109 16754 2 . ASP 110 110 16754 2 . LYS 111 111 16754 2 . LEU 112 112 16754 2 . LEU 113 113 16754 2 . GLN 114 114 16754 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 16754 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $RPA70A . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 16754 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 16754 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $RPA70A . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . pET15b . . . . . . 16754 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 16754 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system H2O _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Rad51N [U-15N] . . 2 $Rad51N . . . 0.3 0.8 mM . . . . 16754 1 2 RPA70A [U-15N] . . 1 $RPA70A . . . 0.3 0.8 mM . . . . 16754 1 3 Tis 'natural abundance' . . . . . . 10 . . mM . . . . 16754 1 4 'Potassium Chloride' 'natural abundance' . . . . . . 20 . . mM . . . . 16754 1 5 BME 'natural abundance' . . . . . . 1 . . mM . . . . 16754 1 6 'sodium azide' 'natural abundance' . . . . . . 1 . . uM . . . . 16754 1 7 H2O 'natural abundance' . . . . . . 100 . . % . . . . 16754 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 16754 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7.0 . pH 16754 1 pressure 1 . atm 16754 1 temperature 300 . K 16754 1 stop_ save_ ############################ # Computer software used # ############################ save_Felix _Software.Sf_category software _Software.Sf_framecode Felix _Software.Entry_ID 16754 _Software.ID 1 _Software.Name FELIX _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Accelrys Software Inc.' . . 16754 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 16754 1 stop_ save_ save_HADDOCK _Software.Sf_category software _Software.Sf_framecode HADDOCK _Software.Entry_ID 16754 _Software.ID 2 _Software.Name HADDOCK _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Dominguez, C., Boelens, R., and Bonvin, A.M. 2003' . . 16754 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'structure solution' 16754 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 16754 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 16754 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker Avance . 800 . . . 16754 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 16754 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 16754 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 16754 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 16754 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $Felix . . 16754 1 2 $HADDOCK . . 16754 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kd 180 59 uM 16754 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 RPA70A 1 $RPA70A 16754 1 1 1 2 Rad51N 2 $Rad51N 16754 1 stop_ save_