BMRB Entry 16659

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16659

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Title:
Assignment and structural characterization of intrinsically disordered CDK inhibitor phosphoSic1 from yeast
Deposition date:
2009-12-30
Original release date:
2010-05-05
Authors:
Mittag, Tanja; Choy, Wing-Yiu; Marsh, Joseph; Orlicky, Stephen; Grishaev, Alexander; Lin, Hong; Sicheri, Frank; Tyers, Mike; Forman-Kay, Julie
Citation:

Citation: Mittag, Tanja; Marsh, Joseph; Grishaev, Alexander; Orlicky, Stephen; Lin, Hong; Sicheri, Frank; Tyers, Mike; Forman-Kay, Julie. "Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase."  Structure (Cambridge, MA, U. S.) 18, 494-506 (2010).
PubMed: 20399186

Assembly members:

Assembly members:
pSic1, polymer, 92 residues, 10195 Da.
pSic1_G-1X, polymer, 92 residues, Formula weight is not available
pSic1_N21X, polymer, 92 residues, Formula weight is not available
pSic1_S38X, polymer, 92 residues, Formula weight is not available
pSic1_N64X, polymer, 92 residues, Formula weight is not available
pSic1_P83X, polymer, 92 residues, Formula weight is not available
pSic1_T90X, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pSic1: GSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFPKSSVKRT
pSic1_G-1X: XSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFPKSSVKRT
pSic1_N21X: GSMTPSXPPRSRGTRYLAQP SGXTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFPKSSVKRT
pSic1_S38X: GSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPX QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFPKSSVKRT
pSic1_N64X: GSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSXMGMTXPFNGLTXPQ RXPFPKSSVKRT
pSic1_P83X: GSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFXKSSVKRT
pSic1_T90X: GSMTPSXPPRSRGTRYLAQP SGNTSSSALMQGQKXPQKPS QNLVPVXPSTTKSFKNAPLL APPNSNMGMTXPFNGLTXPQ RXPFPKSSVKRX

Data sets:
Data typeCount
13C chemical shifts171
15N chemical shifts75
1H chemical shifts75
heteronuclear NOE values74
residual dipolar couplings63
T1 relaxation values74
T2 relaxation values74

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pSic11

Entities:

Entity 1, pSic1 92 residues - 10195 Da.

The first two residues represent a non-native artifact from cleavage of an affinity tag and are therefore numbered -1 and 0. Residue M1 is the actual beginning of the native Sic1 sequence. The construct used represents the first 90 residues of Sic1, the so-called targeting region. Residues 5, 33 and 45 are phosphoThr, 69,76 and 80 phosphoSer.

1   GLYSERMETTHRPROSERTPOPROPROARG
2   SERARGGLYTHRARGTYRLEUALAGLNPRO
3   SERGLYASNTHRSERSERSERALALEUMET
4   GLNGLYGLNLYSTPOPROGLNLYSPROSER
5   GLNASNLEUVALPROVALTPOPROSERTHR
6   THRLYSSERPHELYSASNALAPROLEULEU
7   ALAPROPROASNSERASNMETGLYMETTHR
8   SEPPROPHEASNGLYLEUTHRSEPPROGLN
9   ARGSEPPROPHEPROLYSSERSERVALLYS
10   ARGTHR

Related Database Links:

UNP P38634

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks