BMRB Entry 16581

Title:
2J coupling constants in human Frataxin C-terminal domain (90-210)   PubMed: 20131375
Deposition date:
2009-10-25
Original release date:
2010-02-08
Authors:
Schmidt, Jurgen; Lohr, Frank
Citation:

Citation: Schmidt, Jurgen; Hua, Yixun; Lohr, Frank. "Correlation of (2)J couplings with protein secondary structure."  Proteins 78, 1544-1562 (2010).

Assembly members:

Assembly members:
Frataxin, polymer, 121 residues, 13492.6 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET

Data sets:
Data typeCount
coupling constants223

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 121 residues - 13492.6 Da.

1   METASPGLUTHRTHRTYRGLUARGLEUALA
2   GLUGLUTHRLEUASPSERLEUALAGLUPHE
3   PHEGLUASPLEUALAASPLYSPROTYRTHR
4   PHEGLUASPTYRASPVALSERPHEGLYSER
5   GLYVALLEUTHRVALLYSLEUGLYGLYASP
6   LEUGLYTHRTYRVALILEASNLYSGLNTHR
7   PROASNLYSGLNILETRPLEUSERSERPRO
8   SERSERGLYPROLYSARGTYRASPTRPTHR
9   GLYLYSASNTRPVALTYRSERHISASPGLY
10   VALSERLEUHISGLULEULEUALAALAGLU
11   LEUTHRLYSALALEULYSTHRLYSLEUASP
12   LEUSERSERLEUALATYRSERGLYLYSASP
13   ALA

Samples:

sample_1: Frataxin, [U-95% 13C; U-95% 15N], 1.7 mM; TRIS 20 mM; sodium chloride 100 mM; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D IPAP-type HN(CO-a/b-NCa-J)-TROSYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

jeval, JM Schmidt - coupling constant extraction, data analysis, multiplet simulation

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15233 15234 15235 15736 15906 4342
PDB
GB AAA98508 AAA98509 AAA98510 AAH23633 AAH48097
REF NP_000135 NP_001155178 NP_001247670 NP_852090 XP_001137864
SP Q16595
AlphaFold Q16595