BMRB Entry 15906

Title:
1J coupling constants related to the Ca carbons in human Frataxin C-terminal domain (90-210)   PubMed: 18853398
Deposition date:
2008-08-04
Original release date:
2008-11-19
Authors:
Schmidt, Jurgen; Lohr, Frank
Citation:

Citation: Schmidt, Jurgen; Howard, Mark; Maestre-Martinez, Mitcheell; Perez, Carlos; Lohr, Frank. "Variation in protein C(alpha)-related one-bond J couplings"  Magn. Reson. Chem. 47, 16-30 (2009).

Assembly members:

Assembly members:
Frataxin, polymer, 121 residues, 13492.6 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET

Data sets:
Data typeCount
coupling constants425

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 121 residues - 13492.6 Da.

1   METASPGLUTHRTHRTYRGLUARGLEUALA
2   GLUGLUTHRLEUASPSERLEUALAGLUPHE
3   PHEGLUASPLEUALAASPLYSPROTYRTHR
4   PHEGLUASPTYRASPVALSERPHEGLYSER
5   GLYVALLEUTHRVALLYSLEUGLYGLYASP
6   LEUGLYTHRTYRVALILEASNLYSGLNTHR
7   PROASNLYSGLNILETRPLEUSERSERPRO
8   SERSERGLYPROLYSARGTYRASPTRPTHR
9   GLYLYSASNTRPVALTYRSERHISASPGLY
10   VALSERLEUHISGLULEULEUALAALAGLU
11   LEUTHRLYSALALEULYSTHRLYSLEUASP
12   LEUSERSERLEUALATYRSERGLYLYSASP
13   ALA

Samples:

doubly_labeled: Frataxin, [U-95% 13C; U-95% 15N], 1.7 mM; TRIS 20 mM; sodium chloride 100 mM; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D Ca-coupled HNCOdoubly_labeledisotropicsample_conditions_1
2D IPAP-type HN(CO-a/b-NCa-J)-TROSYdoubly_labeledisotropicsample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-HN(CO)CAdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CAdoubly_labeledisotropicsample_conditions_1
2D HN(COCA-J)doubly_labeledisotropicsample_conditions_1
2D Ha-coupled HN(CO)CAdoubly_labeledisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

jeval, JM Schmidt - coupling constant extraction, data analysis, multiplet simulation

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15233 15234 15235 15736 16581 4342
PDB
GB AAA98508 AAA98509 AAA98510 AAH23633 AAH48097
REF NP_000135 NP_001155178 NP_001247670 NP_852090 XP_001137864
SP Q16595
AlphaFold Q16595