BMRB Entry 15736
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15736
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Citation: Kondapalli, Kalyan; Bencze, Krisztina; Dizen, Eric; Cowan, James; Stemmler, Timothy. "1H, C13, 15N Resonace assignments for full-length human frataxin" J. Biomol. NMR ., .-..
Assembly members:
Full-length_Human_frataxin, polymer, 187 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28b
Entity Sequences (FASTA):
Full-length_Human_frataxin: MGSSHHHHHHSSGLVPRGSH
MDIDATCTPRRASSNQRGLN
QIWNVKKQSVYLMNLRKSGT
LGHPGSLDETTYERLAEETL
DSLAEFFEDLADKPYTFEDY
DVSFGSGVLTVKLGGDLGTY
VINKQTPNKQIWLSSPSSGP
KRYDWTGKNWVYSHDGVSLH
ELLAAELTKALKTKLDLSSL
AYSGKDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 584 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 669 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Full-length human frataxin | 1 |
Entities:
Entity 1, Full-length human frataxin 187 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ASP | ILE | ASP | ALA | THR | CYS | THR | PRO | ARG | ||||
| 4 | ARG | ALA | SER | SER | ASN | GLN | ARG | GLY | LEU | ASN | ||||
| 5 | GLN | ILE | TRP | ASN | VAL | LYS | LYS | GLN | SER | VAL | ||||
| 6 | TYR | LEU | MET | ASN | LEU | ARG | LYS | SER | GLY | THR | ||||
| 7 | LEU | GLY | HIS | PRO | GLY | SER | LEU | ASP | GLU | THR | ||||
| 8 | THR | TYR | GLU | ARG | LEU | ALA | GLU | GLU | THR | LEU | ||||
| 9 | ASP | SER | LEU | ALA | GLU | PHE | PHE | GLU | ASP | LEU | ||||
| 10 | ALA | ASP | LYS | PRO | TYR | THR | PHE | GLU | ASP | TYR | ||||
| 11 | ASP | VAL | SER | PHE | GLY | SER | GLY | VAL | LEU | THR | ||||
| 12 | VAL | LYS | LEU | GLY | GLY | ASP | LEU | GLY | THR | TYR | ||||
| 13 | VAL | ILE | ASN | LYS | GLN | THR | PRO | ASN | LYS | GLN | ||||
| 14 | ILE | TRP | LEU | SER | SER | PRO | SER | SER | GLY | PRO | ||||
| 15 | LYS | ARG | TYR | ASP | TRP | THR | GLY | LYS | ASN | TRP | ||||
| 16 | VAL | TYR | SER | HIS | ASP | GLY | VAL | SER | LEU | HIS | ||||
| 17 | GLU | LEU | LEU | ALA | ALA | GLU | LEU | THR | LYS | ALA | ||||
| 18 | LEU | LYS | THR | LYS | LEU | ASP | LEU | SER | SER | LEU | ||||
| 19 | ALA | TYR | SER | GLY | LYS | ASP | ALA |
Samples:
sample_1: Full-length Human frataxin, [U-15N], 1 mM; potassium phosphate, [U-13C], 100 mM; D2O 30%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 15233 15234 15235 15906 16581 4342 |
| PDB | |
| GB | AAA98508 AAA98509 AAA98510 AAH23633 AAH48097 |
| REF | NP_000135 NP_001155178 NP_852090 XP_010344443 XP_011804278 |
| SP | Q16595 |
| AlphaFold | Q16595 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks