BMRB Entry 15736

Title:
Full length human frataxin
Deposition date:
2008-04-17
Original release date:
2009-10-12
Authors:
Kondapalli, Kalyan; Bencze, Krisztina; Dizen, Eric; Cowan, James; Stemmler, Timothy
Citation:

Citation: Kondapalli, Kalyan; Bencze, Krisztina; Dizen, Eric; Cowan, James; Stemmler, Timothy. "1H, C13, 15N Resonace assignments for full-length human frataxin"  J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
Full-length_Human_frataxin, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 28b

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 28b

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts147
1H chemical shifts669

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Full-length human frataxin1

Entities:

Entity 1, Full-length human frataxin 187 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASPILEASPALATHRCYSTHRPROARG
4   ARGALASERSERASNGLNARGGLYLEUASN
5   GLNILETRPASNVALLYSLYSGLNSERVAL
6   TYRLEUMETASNLEUARGLYSSERGLYTHR
7   LEUGLYHISPROGLYSERLEUASPGLUTHR
8   THRTYRGLUARGLEUALAGLUGLUTHRLEU
9   ASPSERLEUALAGLUPHEPHEGLUASPLEU
10   ALAASPLYSPROTYRTHRPHEGLUASPTYR
11   ASPVALSERPHEGLYSERGLYVALLEUTHR
12   VALLYSLEUGLYGLYASPLEUGLYTHRTYR
13   VALILEASNLYSGLNTHRPROASNLYSGLN
14   ILETRPLEUSERSERPROSERSERGLYPRO
15   LYSARGTYRASPTRPTHRGLYLYSASNTRP
16   VALTYRSERHISASPGLYVALSERLEUHIS
17   GLULEULEUALAALAGLULEUTHRLYSALA
18   LEULYSTHRLYSLEUASPLEUSERSERLEU
19   ALATYRSERGLYLYSASPALA

Samples:

sample_1: Full-length Human frataxin, [U-15N], 1 mM; potassium phosphate, [U-13C], 100 mM; D2O 30%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15233 15234 15235 15906 16581 4342
PDB
GB AAA98508 AAA98509 AAA98510 AAH23633 AAH48097
REF NP_000135 NP_001155178 NP_852090 XP_010344443 XP_011804278
SP Q16595
AlphaFold Q16595

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks