BMRB Entry 16471

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for IQGAP1 (401-533)
Published: 2010-01-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16471

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for IQGAP1 (401-533)

Deposition date:

2009-08-27

Original release date:

2010-01-28

Authors:

Smith, Matthew; Ikura, Mitsu

Citation:

Citation: Smith, Matthew; Hardy, W. Rod; Li, Guang-Yao; Goudreault, Marilyn; Hersch, Steven; Metalnikov, Pavel; Starostine, Andrei; Pawson, Tony; Ikura, Mitsuhiko. "The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1." EMBO J. 29, 884-896 (2010).
PubMed: 20075861

Assembly members:

Assembly members:
IQGAP1_(401-533), polymer, 133 residues, 14888 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IQGAP1_(401-533): KGIAEKTVLELMNPEAQLPQ VYPFAADLYQKELATLQQQS PEHSLTHPELTVAVEMLSSV ALINRALESGDMTTVWKQLS SSVTGLTNIEEENCQRYLDE LMKLKAQAHAENNAFITWND IQACVDHVNLVVH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	237
15N chemical shifts	115
1H chemical shifts	115

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ShcA PTB Domain Ligand	1

Entities:

Entity 1, ShcA PTB Domain Ligand 133 residues - 14888 Da.

1

LYS

GLY

ILE

ALA

GLU

LYS

THR

VAL

LEU

GLU

2

LEU

MET

ASN

PRO

GLU

ALA

GLN

LEU

PRO

GLN

3

VAL

TYR

PRO

PHE

ALA

ASP

LEU

TYR

GLN

4

LYS

GLU

LEU

ALA

THR

LEU

GLN

SER

5

PRO

GLU

HIS

SER

LEU

THR

HIS

PRO

GLU

LEU

6

THR

VAL

ALA

VAL

GLU

MET

LEU

SER

VAL

7

ALA

LEU

ILE

ASN

ARG

ALA

LEU

GLU

SER

GLY

8

ASP

MET

THR

VAL

TRP

LYS

GLN

LEU

SER

9

SER

VAL

THR

GLY

LEU

THR

ASN

ILE

GLU

10

GLU

ASN

CYS

GLN

ARG

TYR

LEU

ASP

GLU

11

LEU

MET

LYS

LEU

LYS

ALA

GLN

ALA

HIS

ALA

12

GLU

ASN

ALA

PHE

ILE

THR

TRP

ASN

ASP

13

ILE

GLN

ALA

CYS

VAL

ASP

HIS

VAL

ASN

LEU

14

VAL

HIS

Samples:

sample_1: IQGAP1 (401-533), [U-13C; U-15N], 200 uM; D2O 10%; H2O 90%; NaCl 100 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

SWS	Q9JKF1
DBJ	BAC97854 BAE37174 BAE37805
GB	AAF60344 AAH22105 AAH37685 AAH46385 EDL06977
REF	NP_001101959 NP_057930 XP_003513458 XP_005077870 XP_005357708
SP	Q9JKF1
AlphaFold	Q9JKF1