BMRB Entry 16058
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PDB ID: 2kc0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16058
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Cantini, Francesca; Veggi, Daniele; Dragonetti, Sara; Savino, Silvana; Scarselli, Maria; Romagnoli, Giacomo; Pizza, Mariagrazia; Banci, Lucia; Rappuoli, Rino. "Solution Structure of the Factor H-binding Protein, a Survival Factor and Protective Antigen of Neisseria meningitidis" J. Biol. Chem. 284, 9022-9026 (2009).
PubMed: 19196709
Assembly members:
Factor H-binding Protein, polymer, 255 residues, 25971.215 Da.
Natural source: Common Name: Neisseria meningitidis Taxonomy ID: 487 Superkingdom: Bacteria Kingdom: not available Genus/species: Neisseria meningitidis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET20b+
Entity Sequences (FASTA):
Factor H-binding Protein: MVAADIGAGLADALTAPLDH
KDKGLQSLTLDQSVRKNEKL
KLAAQGAEKTYGNGDSLNTG
KLKNDKVSRFDFIRQIEVDG
QLITLESGEFQVYKQSHSAL
TAFQTEQIQDSEHSGKMVAK
RQFRIGDIAGEHTSFDKLPE
GGRATYRGTAFGSDDAGGKL
TYTIDFAAKQGNGKIEHLKS
PELNVDLAAADIKPDGKRHA
VISGSVLYNQAEKGSYSLGI
FGGKAQEVAGSAEVKTVNGI
RHIGLAAKQHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 932 |
| 15N chemical shifts | 257 |
| 1H chemical shifts | 1629 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Factor H-binding Protein | 1 |
Entities:
Entity 1, Factor H-binding Protein 255 residues - 25971.215 Da.
Residues 1-7 represent a non-structured region and it has not been submitted to PDB. Residues 255-261 represent a non-native affinity tag
| 1 | MET | VAL | ALA | ALA | ASP | ILE | GLY | ALA | GLY | LEU | ||||
| 2 | ALA | ASP | ALA | LEU | THR | ALA | PRO | LEU | ASP | HIS | ||||
| 3 | LYS | ASP | LYS | GLY | LEU | GLN | SER | LEU | THR | LEU | ||||
| 4 | ASP | GLN | SER | VAL | ARG | LYS | ASN | GLU | LYS | LEU | ||||
| 5 | LYS | LEU | ALA | ALA | GLN | GLY | ALA | GLU | LYS | THR | ||||
| 6 | TYR | GLY | ASN | GLY | ASP | SER | LEU | ASN | THR | GLY | ||||
| 7 | LYS | LEU | LYS | ASN | ASP | LYS | VAL | SER | ARG | PHE | ||||
| 8 | ASP | PHE | ILE | ARG | GLN | ILE | GLU | VAL | ASP | GLY | ||||
| 9 | GLN | LEU | ILE | THR | LEU | GLU | SER | GLY | GLU | PHE | ||||
| 10 | GLN | VAL | TYR | LYS | GLN | SER | HIS | SER | ALA | LEU | ||||
| 11 | THR | ALA | PHE | GLN | THR | GLU | GLN | ILE | GLN | ASP | ||||
| 12 | SER | GLU | HIS | SER | GLY | LYS | MET | VAL | ALA | LYS | ||||
| 13 | ARG | GLN | PHE | ARG | ILE | GLY | ASP | ILE | ALA | GLY | ||||
| 14 | GLU | HIS | THR | SER | PHE | ASP | LYS | LEU | PRO | GLU | ||||
| 15 | GLY | GLY | ARG | ALA | THR | TYR | ARG | GLY | THR | ALA | ||||
| 16 | PHE | GLY | SER | ASP | ASP | ALA | GLY | GLY | LYS | LEU | ||||
| 17 | THR | TYR | THR | ILE | ASP | PHE | ALA | ALA | LYS | GLN | ||||
| 18 | GLY | ASN | GLY | LYS | ILE | GLU | HIS | LEU | LYS | SER | ||||
| 19 | PRO | GLU | LEU | ASN | VAL | ASP | LEU | ALA | ALA | ALA | ||||
| 20 | ASP | ILE | LYS | PRO | ASP | GLY | LYS | ARG | HIS | ALA | ||||
| 21 | VAL | ILE | SER | GLY | SER | VAL | LEU | TYR | ASN | GLN | ||||
| 22 | ALA | GLU | LYS | GLY | SER | TYR | SER | LEU | GLY | ILE | ||||
| 23 | PHE | GLY | GLY | LYS | ALA | GLN | GLU | VAL | ALA | GLY | ||||
| 24 | SER | ALA | GLU | VAL | LYS | THR | VAL | ASN | GLY | ILE | ||||
| 25 | ARG | HIS | ILE | GLY | LEU | ALA | ALA | LYS | GLN | HIS | ||||
| 26 | HIS | HIS | HIS | HIS | HIS |
Related Database Links:
| PDB | 1YS5 2KC0 2W80 2W81 2YPV 3KVD 4AYD 4AYE |
| EMBL | CCA43599 |
| GB | AAF42204 AAR84468 AAR84469 AAR84470 AAR84471 |
| REF | NP_274866 WP_002225785 WP_002255070 WP_010981001 WP_039854169 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks