BMRB Entry 15898
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15898
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Citation: Santini, Guillaume; Cognet, Jean; Xu, Duanxiang; Singarapu, Kiran; Herve du Penhoat, Catherine. "Nucleic Acid Folding Determined by Mesoscale Modeling and NMR Spectroscopy: Solution Structure of d(GCGAAAGC)" J. Phys. Chem. B 113, 6881-6893 (2009).
PubMed: 19374420
Assembly members:
dGCGAAAGC, polymer, 8 residues, 2443.6 Da.
Natural source: Common Name: phage G4 Taxonomy ID: 10843 Superkingdom: Viruses Kingdom: not available Genus/species: Microvirus Enterobacteria phage G4 sensu lato
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
dGCGAAAGC: GCGAAAGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 42 |
| 1H chemical shifts | 72 |
| 31P chemical shifts | 7 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain A | 1 |
Entities:
Entity 1, chain A 8 residues - 2443.6 Da.
| 1 | DG | DC | DG | DA | DA | DA | DG | DC |
Samples:
sample_1: dGCGAAAGC 4 mM; sodium phosphate 10 mM; sodium azide 0.0025 mM; EDTA 0.005 mM; sodium chloride 50 mM; D2O 100%
sample_2: dGCGAAAGC 4 mM; sodium phosphate 10 mM; sodium azide 0.0025 mM; EDTA 0.005 mM; sodium chloride 50 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298.15 K
sample_conditions_2: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 274.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-31P COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
VNMR, Varian - collection
PROSA, Guntert - data analysis
DYANA, Guntert, Braun and Wuthrich - refinement, structure solution
Molmol, Koradi, Billeter and Wuthrich - visualisation
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian Unity 400 MHz