BMRB Entry 15884

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CTL9 native state at pH 3.8
Published: 2008-09-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15884

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CTL9 native state at pH 3.8

Deposition date:

2008-07-24

Original release date:

2008-09-19

Authors:

Shan, Bing; Raleigh, Daniel

Citation:

Citation: Shan, Bing; Raleigh, Daniel. "The Low pH Unfolded State of the C-terminal Domain of the Ribosomal Protein L9 Contains Significant Secondary Structure in the Absence of Denaturant but is No More Compact than the Low pH Urea Unfolded State" Biochemistry 47, 9565-9573 (2008).
PubMed: 18707127

Assembly members:

Assembly members:
CTL9, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus stearothermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CTL9: AAEELANAKKLKEQLEKLTV TIPAKAGEGGRLFGSITSKQ IAESLQAQHGLKLDKRKIEL ADAIRALGYTNVPVKLHPEV TATLKVHVTEQK

Data sets:

assigned_chemical_shifts
- CTL9_pH_3.8_native_state

Data type	Count
13C chemical shifts	257
15N chemical shifts	82
1H chemical shifts	270

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTL9	1

Entities:

Entity 1, CTL9 92 residues - Formula weight is not available

CTL9 is the c terminal domain of protein L9, consisting of residues 58-149

1

ALA

GLU

LEU

ALA

ASN

ALA

LYS

2

LEU

LYS

GLU

GLN

LEU

GLU

LYS

LEU

THR

VAL

3

THR

ILE

PRO

ALA

LYS

ALA

GLY

GLU

GLY

4

ARG

LEU

PHE

GLY

SER

ILE

THR

SER

LYS

GLN

5

ILE

ALA

GLU

SER

LEU

GLN

ALA

GLN

HIS

GLY

6

LEU

LYS

LEU

ASP

LYS

ARG

LYS

ILE

GLU

LEU

7

ALA

ASP

ALA

ILE

ARG

ALA

LEU

GLY

TYR

THR

8

ASN

VAL

PRO

VAL

LYS

LEU

HIS

PRO

GLU

VAL

9

THR

ALA

THR

LEU

LYS

VAL

HIS

VAL

THR

GLU

10

GLN

LYS

Samples:

sample_1: CTL9, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium acetate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 120 mM; pH: 3.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	15883 17460
PDB	1DIV 1GIY 1YL3 2B66 2B9N 2B9P 487D
EMBL	CAA43972
GB	AAA22701 AKM20686 AKU26730 KFL16938 KFX34457
PRF	0701226A 1714237A
REF	WP_033008865 WP_050368025
SP	P02417
AlphaFold	P02417