BMRB Entry 15883

Name: Backbone and 13C and 1H assignments for acid unfolded state and urea unfolded state of CTL9
Published: 2008-09-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15883

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and 13C and 1H assignments for acid unfolded state and urea unfolded state of CTL9

Deposition date:

2008-07-24

Original release date:

2008-09-19

Authors:

Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel

Citation:

Citation: Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel. "The Low pH Unfolded State of the C-terminal Domain of the Ribosomal Protein L9 Contains Significant Secondary Structure in the Absence of Denaturant but is No More Compact than the Low pH Urea Unfolded State" Biochemistry 47, 9565-9573 (2008).
PubMed: 18707127

Assembly members:

Assembly members:
CTL9, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus stearothermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CTL9: AAEELANAKKLKEQLEKLTV TIPAKAGEGGRLFGSITSKQ IAESLQAQHGLKLDKRKIEL ADAIRALGYTNVPVKLHPEV TATLKVHVTEQK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	267
15N chemical shifts	88
1H chemical shifts	290

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTL9	1

Entities:

Entity 1, CTL9 92 residues - Formula weight is not available

1

ALA

GLU

LEU

ALA

ASN

ALA

LYS

2

LEU

LYS

GLU

GLN

LEU

GLU

LYS

LEU

THR

VAL

3

THR

ILE

PRO

ALA

LYS

ALA

GLY

GLU

GLY

4

ARG

LEU

PHE

GLY

SER

ILE

THR

SER

LYS

GLN

5

ILE

ALA

GLU

SER

LEU

GLN

ALA

GLN

HIS

GLY

6

LEU

LYS

LEU

ASP

LYS

ARG

LYS

ILE

GLU

LEU

7

ALA

ASP

ALA

ILE

ARG

ALA

LEU

GLY

TYR

THR

8

ASN

VAL

PRO

VAL

LYS

LEU

HIS

PRO

GLU

VAL

9

THR

ALA

THR

LEU

LYS

VAL

HIS

VAL

THR

GLU

10

GLN

LYS

Samples:

sample_1: CTL9, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 700 MHz

BMRB	15884 17460
PDB	1DIV 1GIY 1YL3 2B66 2B9N 2B9P 487D
EMBL	CAA43972
GB	AAA22701 AKM20686 AKU26730 KFL16938 KFX34457
PRF	0701226A 1714237A
REF	WP_033008865 WP_050368025
SP	P02417
AlphaFold	P02417