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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15776
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Amero, Carlos; Boomershine, William; Xu, Yiren; Foster, Mark. "Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner" Biochemistry 47, 11704-11710 (2008).
PubMed: 18922021
Assembly members:
Rpp21, polymer, 123 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-33b
Entity Sequences (FASTA):
Rpp21: MAKYNEKKEKKRIAKERIDI
LFSLAERVFPYSPELAKRYV
ELALLVQQKAKVKIPRKWKR
RYCKKCHAFLVPGINARVRL
RQKRMPHIVVKCLECGHIMR
YPYIKEIKKRRKEKMEYGGL
VPR
Data type | Count |
13C chemical shifts | 370 |
15N chemical shifts | 75 |
1H chemical shifts | 558 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Rpp21 | 1 |
2 | Zn | 2 |
Entity 1, Rpp21 123 residues - 65.409 Da.
1 | MET | ALA | LYS | TYR | ASN | GLU | LYS | LYS | GLU | LYS | ||||
2 | LYS | ARG | ILE | ALA | LYS | GLU | ARG | ILE | ASP | ILE | ||||
3 | LEU | PHE | SER | LEU | ALA | GLU | ARG | VAL | PHE | PRO | ||||
4 | TYR | SER | PRO | GLU | LEU | ALA | LYS | ARG | TYR | VAL | ||||
5 | GLU | LEU | ALA | LEU | LEU | VAL | GLN | GLN | LYS | ALA | ||||
6 | LYS | VAL | LYS | ILE | PRO | ARG | LYS | TRP | LYS | ARG | ||||
7 | ARG | TYR | CYS | LYS | LYS | CYS | HIS | ALA | PHE | LEU | ||||
8 | VAL | PRO | GLY | ILE | ASN | ALA | ARG | VAL | ARG | LEU | ||||
9 | ARG | GLN | LYS | ARG | MET | PRO | HIS | ILE | VAL | VAL | ||||
10 | LYS | CYS | LEU | GLU | CYS | GLY | HIS | ILE | MET | ARG | ||||
11 | TYR | PRO | TYR | ILE | LYS | GLU | ILE | LYS | LYS | ARG | ||||
12 | ARG | LYS | GLU | LYS | MET | GLU | TYR | GLY | GLY | LEU | ||||
13 | VAL | PRO | ARG |
Entity 2, Zn - Zn - 65.409 Da.
1 | ZN |
sample_1: TRIS, [U-99% 2H], 10 mM; potassium chloride 10 mM; sodium azide 0.02%; Rpp21, [U-100% 13C; U-100% 15N], 1 mM; ZINC chloride 0.3 mM; H2O 90%; D2O 10%
sample_complex: TRIS, [U-99% 2H], 10 mM; potassium chloride 10 mM; sodium azide 0.02%; Rpp21, [U-100% 13C; U-100% 15N], 0.5 mM; ZINC chloride 0.3 mM; Rpp29 0.5 mM; H2O 90%; D2O 10%
sample_2: TRIS, [U-99% 2H], 10 mM; potassium chloride 10 mM; sodium azide 0.02%; Rpp21, [U-100% 13C; U-100% 15N], 1 mM; ZINC chloride 0.3 mM; H2O 90%; D2O 10%
sample_cobalt: TRIS, [U-99% 2H], 10 mM; potassium chloride 10 mM; sodium azide 0.02%; Rpp21, [U-100% 13C; U-100% 15N], 1 mM; cobalt chloride 0.3 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 6.7; pressure: 1 atm; temperature: 323 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_complex | isotropic | sample_conditions_1 |
3D HNCO | sample_complex | isotropic | sample_conditions_1 |
3D HNCA | sample_complex | isotropic | sample_conditions_1 |
3D HNCACB | sample_complex | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_complex | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_cobalt | isotropic | sample_conditions_1 |
3D HNCO | sample_cobalt | isotropic | sample_conditions_1 |
3D HNCA | sample_cobalt | isotropic | sample_conditions_1 |
3D HNCACB | sample_cobalt | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_cobalt | isotropic | sample_conditions_1 |
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement, structure solution
NMRView, Johnson, One Moon Scientific - data analysis, structure solution
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, structure solution
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks