BMRB Entry 15708
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15708
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Citation: Zou, Chao; Kumaran, Sowmini; Markovic, Stefan; Walser, Reto; Zerbe, Oliver. "Studies of the Structure of the N-Terminal Domain from the Y4 Receptor-a G Protein-Coupled Receptor-and its Interaction with Hormones from the NPY Family" ChemBioChem 9, 2276-2284 (2008).
PubMed: 18767100
Assembly members:
N-terminal_domain_of_Y4_receptor, polymer, 41 residues, 4561.191 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
N-terminal_domain_of_Y4_receptor: MNTSHLLALLLPKSPQGENR
SKPLGTPYNFSEHCQDSVDV
M
- assigned_chemical_shifts
- heteronucl_NOEs
| Data type | Count |
| 1H chemical shifts | 537 |
| heteronuclear NOE values | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 41 residues - 4561.191 Da.
| 1 | MET | ASN | THR | SER | HIS | LEU | LEU | ALA | LEU | LEU | ||||
| 2 | LEU | PRO | LYS | SER | PRO | GLN | GLY | GLU | ASN | ARG | ||||
| 3 | SER | LYS | PRO | LEU | GLY | THR | PRO | TYR | ASN | PHE | ||||
| 4 | SER | GLU | HIS | CYS | GLN | ASP | SER | VAL | ASP | VAL | ||||
| 5 | MET |
Samples:
sample_1: N-terminal domain of Y4 receptor 1 mM; MES 40 mM; SDS 300 mM
sample_2: N-terminal domain of Y4 receptor 1 mM; MES 40 mM; DPC 300 mM
sample_conditions_1: ionic strength: 0 M; pH: 5.6; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
XEASY/CARA v1.5, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz