BMRB Entry 15436

Title:
alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR
Deposition date:
2007-08-16
Original release date:
2008-03-13
Authors:
Feng, Zhi-Ping; Ellison, Michael
Citation:

Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and Identification of Key Receptor-Binding Residues."  J. Mol. Biol. 337, 1216-1227 (2008).
PubMed: 18295795

Assembly members:

Assembly members:
alpha-RgIA, polymer, 13 residues, 1580.875 Da.

Natural source:

Natural source:   Common Name: Conus regius   Taxonomy ID: 101314   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus regius

Experimental source:

Experimental source:   Production method: chemical synthesis   Vector: F-moc

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha-RgIA: GCCSDVRCRYRCR

Data sets:
Data typeCount
1H chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-RgIA1

Entities:

Entity 1, alpha-RgIA 13 residues - 1580.875 Da.

1   GLYCYSCYSSERASPVALARGCYSARGTYR
2   ARGCYSARG

Samples:

sample_1: alpha-RgIA 1.0 ± 0.2 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 4.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Bruker DRX 600 MHz