BMRB Entry 15350

Name: Northeast Structural Genomics Target SR478
Published: 2007-07-31

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PDB ID: 2js1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15350
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Northeast Structural Genomics Target SR478

Deposition date:

2007-06-29

Original release date:

2007-07-31

Authors:

Macnaughtan, Megan; Weldeghiorghis, Thomas; Wang, Xu; Bansal, Sonal; Tian, Fang; Wang, Dongyan; Janjua, Haleema; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Prestegard, James

Citation:

Citation: Macnaughtan, Megan; Weldeghiorghis, Thomas; Wang, Xu; Bansal, Sonal; Tian, Fang; Wang, Dongyan; Janjua, Haleema; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Prestegard, James. "NMR Structure of the Bacillus subtilis Protein YvfG, Northeast Structural Genomics Target SR478" .

Assembly members:

Assembly members:
SR478, polymer, 80 residues, 9556.924 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SR478: MSELFSVPYFIENLKQHIEM NQSEDKIHAMNSYYRSVVST LVQDQLTKNAVVLKRIQHLD EAYNKVKRGESKLEHHHHHH

Data sets:

RDCs
- RDC_list_1
assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	244
15N chemical shifts	72
1H chemical shifts	421
residual dipolar couplings	52

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SR478, subunit 1	1
2	SR478, subunit 2	1

Entities:

Entity 1, SR478, subunit 1 80 residues - 9556.924 Da.

1	MET	SER	GLU	LEU	PHE	SER	VAL	PRO	TYR	PHE
2	ILE	GLU	ASN	LEU	LYS	GLN	HIS	ILE	GLU	MET
3	ASN	GLN	SER	GLU	ASP	LYS	ILE	HIS	ALA	MET
4	ASN	SER	TYR	TYR	ARG	SER	VAL	VAL	SER	THR
5	LEU	VAL	GLN	ASP	GLN	LEU	THR	LYS	ASN	ALA
6	VAL	VAL	LEU	LYS	ARG	ILE	GLN	HIS	LEU	ASP
7	GLU	ALA	TYR	ASN	LYS	VAL	LYS	ARG	GLY	GLU
8	SER	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1_NC	isotropic	sample_conditions_1
3D 1H-13C NOESY ALIPH	sample_1_NC	isotropic	sample_conditions_1
3D 1H-13C NOESY ALIPH NC-14N	sample_2_NC-14N	isotropic	sample_conditions_1
3D HNCO	sample_1_NC	isotropic	sample_conditions_1
3D 1H-13C NOESY AROMATIC	sample_1_NC	isotropic	sample_conditions_1
3D HNCACB	sample_1_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC ALIPH	sample_1_NC	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC ALIPH 13C-Coupled	sample_3_NC5	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1_NC	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1_NC	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1_NC	isotropic	sample_conditions_1
3D C(CO)NH	sample_1_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC AROMATIC	sample_1_NC	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2	sample_1_NC	isotropic	sample_conditions_1
2D 1H-15N HSQC RDC	sample_4_NC5-PEG	anisotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 15350

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	SER	GLU	LEU	PHE	SER	VAL	PRO	TYR	PHE
2	ILE	GLU	ASN	LEU	LYS	GLN	HIS	ILE	GLU	MET
3	ASN	GLN	SER	GLU	ASP	LYS	ILE	HIS	ALA	MET
4	ASN	SER	TYR	TYR	ARG	SER	VAL	VAL	SER	THR
5	LEU	VAL	GLN	ASP	GLN	LEU	THR	LYS	ASN	ALA
6	VAL	VAL	LEU	LYS	ARG	ILE	GLN	HIS	LEU	ASP
7	GLU	ALA	TYR	ASN	LYS	VAL	LYS	ARG	GLY	GLU
8	SER	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	GLU	LEU	PHE	SER	VAL	PRO	TYR	PHE
2	ILE	GLU	ASN	LEU	LYS	GLN	HIS	ILE	GLU	MET
3	ASN	GLN	SER	GLU	ASP	LYS	ILE	HIS	ALA	MET
4	ASN	SER	TYR	TYR	ARG	SER	VAL	VAL	SER	THR
5	LEU	VAL	GLN	ASP	GLN	LEU	THR	LYS	ASN	ALA
6	VAL	VAL	LEU	LYS	ARG	ILE	GLN	HIS	LEU	ASP
7	GLU	ALA	TYR	ASN	LYS	VAL	LYS	ARG	GLY	GLU
8	SER	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	GLU	LEU	PHE	SER	VAL	PRO	TYR	PHE
2	ILE	GLU	ASN	LEU	LYS	GLN	HIS	ILE	GLU	MET
3	ASN	GLN	SER	GLU	ASP	LYS	ILE	HIS	ALA	MET
4	ASN	SER	TYR	TYR	ARG	SER	VAL	VAL	SER	THR
5	LEU	VAL	GLN	ASP	GLN	LEU	THR	LYS	ASN	ALA
6	VAL	VAL	LEU	LYS	ARG	ILE	GLN	HIS	LEU	ASP
7	GLU	ALA	TYR	ASN	LYS	VAL	LYS	ARG	GLY	GLU
8	SER	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS