BMRB Entry 15133

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15133
MolProbity Validation Chart

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Title:
Solution NMR structure of protein Uncharacterized BCR, Northeast Structural Genomics Consortium target CgR1
Deposition date:
2007-02-14
Original release date:
2007-03-27
Authors:
Wu, Yibing; Liu, Gaohua; Zhang, Qi; Chen, Chen; Nwosu, Chioma; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Wu, Yibing; Liu, Gaohua; Zhang, Qi; Chen, Chen; Nwosu, Chioma; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of protein Uncharacterized BCR, Northeast Structural Genomics Consortium target CgR1"  .

Assembly members:

Assembly members:
Uncharacterized BCR, polymer, 61 residues, 6989.049 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Uncharacterized BCR: MSLDPQLLEVLACPKDKGPL RYLESEQLLVNERLNLAYRI DDGIPVLLIDEATEWTPNNL E

Data sets:
Data typeCount
13C chemical shifts214
15N chemical shifts63
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Uncharacterized BCR1

Entities:

Entity 1, Uncharacterized BCR 61 residues - 6989.049 Da.

1   METSERLEUASPPROGLNLEULEUGLUVAL
2   LEUALACYSPROLYSASPLYSGLYPROLEU
3   ARGTYRLEUGLUSERGLUGLNLEULEUVAL
4   ASNGLUARGLEUASNLEUALATYRARGILE
5   ASPASPGLYILEPROVALLEULEUILEASP
6   GLUALATHRGLUTRPTHRPROASNASNLEU
7   GLU

Samples:

sample_1: Uncharacterized BCR, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 95%; D2O 5%; NaN3 5%; DTT 100 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM

sample_2: Uncharacterized BCR, [U-5% 13C; U-100% 15N], 1.0 mM; H2O 95%; D2O 5%; NaN3 5%; DTT 100 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYsample_1isotropicsample_conditions_1
GFT (4,3)D HNNCABCAsample_1isotropicsample_conditions_1
GFT (4,3)D CABCA(CO)NHNsample_1isotropicsample_conditions_1
GFT (4,3)D HABCAB(CO)NHNsample_1isotropicsample_conditions_1
GFT (4,3)D HCCHsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY, Bartels et al. - peak picking

CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoAssign, Zimmerman, Moseley, Kulikowski, Montelione - chemical shift assignment

AutoStruct, Huang, Swapana, Rajan, Ke, Xia, Shukla, Inouye and Montelione - structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAB98798 BAF54458
EMBL CAF21416 CCH24571
GB AGN19142 AGN22167 AGT05373 AIK85084 AIK87868
REF NP_600624 WP_003858671 WP_003861608

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks