BMRB Entry 10001

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PDB ID: 2czp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR10001
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A high resolution structure of mastoparan-X strongly bound to lipid-bilayer membrane determined by solid-state NMR

Deposition date:

2005-07-31

Original release date:

2006-06-28

Authors:

Akutsu, Hideo; Fujiwara, Toshimichi; Todokoro, Yasuto

Citation:

Citation: Todokoro, Yasuto; Yumen, Ikuko; Fukushima, Kei; Kang, S.-W.; Park, J.-S.; Kohno, Toshiyuki; Wakamatsu, Kaori; Akutsu, Hideo; Fujiwara, Toshimichi. "Structure of Tightly Membrane-Bound Mastoparan-X, a G-protein-Activating Peptide, Determined by Solid-State NMR" Biophys. J. 91, 1368-1379 (2006).
PubMed: 16714348

Assembly members:

Assembly members:
MP-X, polymer, 14 residues, 1556 Da.

Natural source:

Natural source: Common Name: wasps Taxonomy ID: 7448 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Vespa simillima

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUBK19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MP-X: INWKGIAAMAKKLX

Data sets:

assigned_chemical_shifts
- mpx_assign

Data type	Count
13C chemical shifts	73
15N chemical shifts	20

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MP-X	1

Entities:

Entity 1, MP-X 14 residues - 1556 Da.

1

ILE

ASN

TRP

LYS

GLY

ILE

ALA

MET

ALA

2

LYS

LEU

NLW

Samples:

sample_1: mastoparan-X, [U-95% 13C; U-95% 15N], 5 mg; liposome 50 mg

condition_1: pH: 7.4; temperature: 253 K

condition_2: pH: 7.4; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D N-CA-CO	sample_1	isotropic	condition_1
3D Ni-COi+1-CAi+1	sample_1	isotropic	condition_1
2D RFDR	sample_1	isotropic	condition_1
2D SPCz5	sample_1	isotropic	condition_1
2D (CACB)i+1-(COCA)i	sample_1	isotropic	condition_2
2D Ni-(COCA)i+1	sample_1	isotropic	condition_2
2D N-CA	sample_1	isotropic	condition_2
2D Ni-(COCACB)i+1	sample_1	isotropic	condition_1

Software:

Felix2000 v2000, Accelrys Inc. - assignment

spinsight v4.3.2, VARIAN - processing

NMR spectrometers:

Varian CMX 600 MHz
Varian CMX 500 MHz