BMRB Entry 6268

Title:
CBP TAZ1 Domain
Deposition date:
2004-07-19
Original release date:
2005-02-21
Authors:
De Guzman, Roberto; Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, H. Jane; wright, Peter
Citation:

Citation: De Guzman, Roberto; Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "CBP/p300 TAZ1 domain forms a structured scaffold for ligand binding"  Biochemistry 44, 490-497 (2005).
PubMed: 15641773

Assembly members:

Assembly members:
CBP TAZ1 domain, polymer, 100 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts284
1H chemical shifts562
15N chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP TAZ11
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4ZINC (II) ION, 32

Entities:

Entity 1, CBP TAZ1 100 residues - Formula weight is not available

1   ALATHRGLYPROTHRALAASPPROGLULYS
2   ARGLYSLEUILEGLNGLNGLNLEUVALLEU
3   LEULEUHISALAHISLYSCYSGLNARGARG
4   GLUGLNALAASNGLYGLUVALARGALACYS
5   SERLEUPROHISCYSARGTHRMETLYSASN
6   VALLEUASNHISMETTHRHISCYSGLNALA
7   GLYLYSALACYSGLNVALALAHISCYSALA
8   SERSERARGGLNILEILESERHISTRPLYS
9   ASNCYSTHRARGHISASPCYSPROVALCYS
10   LEUPROLEULYSASNALASERASPLYSARG

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CBP TAZ1 domain, [U-13C; U-15N], 1 mM; Tris 10 mM; NaCl 50 mM; DTT 2 mM

sample_2: CBP TAZ1 domain, [U-15N], 1 mM; Tris 10 mM; NaCl 50 mM; DTT 2 mM

Ex-cond_1: pH: 6.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

SP Q92793 P45481
REF NP_596872 NP_004371 NP_001157494 NP_001073315 NP_001020603
PRF 1923401A
GB AAH72594 AAC51770 AAC51331 AAC08447 AAB28651
DBJ BAI45616 BAG65526 BAE06125
PDB
BMRB 5987 5327 16014

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks