BMRB Entry 16014

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16014
MolProbity Validation Chart

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Title:
Structure of protein complex
Deposition date:
2008-10-30
Original release date:
2009-02-16
Authors:
Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter
Citation:

Citation: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation domains"  EMBO J. 28, 948-958 (2009).
PubMed: 19214187

Assembly members:

Assembly members:
TAZ1, polymer, 100 residues, Formula weight is not available
STAT2, polymer, 57 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAZ1: ATGPTADPEKRKLIQQQLVL LLHAHKCQRREQANGEVRAC SLPHCRTMKNVLNHMTHCQA GKACQVAHCASSRQIISHWK NCTRHDCPVCLPLKNASDKR
STAT2: GSHMVPEPDLPHDLRHLNTE PMEIFRNSVKIEEIMPNGDP LLAGQNTVDEVYVSRPS

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts145
1H chemical shifts1059

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAZ11
2STAT22
3ZINC ION_13
4ZINC ION_23
5ZINC ION_33

Entities:

Entity 1, TAZ1 100 residues - Formula weight is not available

1   ALATHRGLYPROTHRALAASPPROGLULYS
2   ARGLYSLEUILEGLNGLNGLNLEUVALLEU
3   LEULEUHISALAHISLYSCYSGLNARGARG
4   GLUGLNALAASNGLYGLUVALARGALACYS
5   SERLEUPROHISCYSARGTHRMETLYSASN
6   VALLEUASNHISMETTHRHISCYSGLNALA
7   GLYLYSALACYSGLNVALALAHISCYSALA
8   SERSERARGGLNILEILESERHISTRPLYS
9   ASNCYSTHRARGHISASPCYSPROVALCYS
10   LEUPROLEULYSASNALASERASPLYSARG

Entity 2, STAT2 57 residues - Formula weight is not available

1   GLYSERHISMETVALPROGLUPROASPLEU
2   PROHISASPLEUARGHISLEUASNTHRGLU
3   PROMETGLUILEPHEARGASNSERVALLYS
4   ILEGLUGLUILEMETPROASNGLYASPPRO
5   LEULEUALAGLYGLNASNTHRVALASPGLU
6   VALTYRVALSERARGPROSER

Entity 3, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: TAZ1, [U-100% 15N], 1 mM; STAT2; ZINC_ION; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 18650
PDB
DBJ BAE06125 BAG65526 BAI45616
EMBL CDQ85159
GB AAB28651 AAC08447 AAC51331 AAC51770 AAH44677
PRF 1923401A
REF NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001247644
SP P45481 Q6JHU9 Q92793
TPG DAA15549
AlphaFold P45481 Q6JHU9 Q92793

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks