BMRB Entry 5327

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5327
MolProbity Validation Chart

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Title:
Structural basis for Hif-1alpha/CBP recognition in the cellular hypoxic response
Deposition date:
2002-03-19
Original release date:
2002-06-13
Authors:
Dames, Sonja; Martinez-Yamout, Maria; De Guzman, Roberto; Dyson, H. Jane; Wright, Peter
Citation:

Citation: Dames, Sonja; Martinez-Yamout, Maria; De Guzman, Roberto; Dyson, H.; Wright, Peter. "From the Cover: Structural basis for Hif-1alpha/CBP Recognition in the Cellular Hypoxic Response"  Proc. Natl. Acad. Sci. U.S.A. 99, 5271-5276 (2002).
PubMed: 11959977

Assembly members:

Assembly members:
hypoxia induced factor 1 alpha, polymer, 51 residues, Formula weight is not available
CREB-binding protein, polymer, 95 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hypoxia induced factor 1 alpha: SDLACRLLGQSMDESGLPQL TSYDCEVNAPIQGSRNLLQG EELLRALDQVN
CREB-binding protein: ADPEKRKLIQQQLVLLLHAH KCQRREQANGEVRACSLPHC RTMKNVLNHMTHCQAGKACQ VAHCASSRQIISHWKNCTRH DCPVCLPLKNASDKR

Data sets:
Data typeCount
13C chemical shifts467
15N chemical shifts154
1H chemical shifts939

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hif-1alpha1
2CBP2
3Zinc ion, I3
4Zinc ion, II3
5Zinc ion, III3

Entities:

Entity 1, Hif-1alpha 51 residues - Formula weight is not available

1   SERASPLEUALACYSARGLEULEUGLYGLN
2   SERMETASPGLUSERGLYLEUPROGLNLEU
3   THRSERTYRASPCYSGLUVALASNALAPRO
4   ILEGLNGLYSERARGASNLEULEUGLNGLY
5   GLUGLULEULEUARGALALEUASPGLNVAL
6   ASN

Entity 2, CBP 95 residues - Formula weight is not available

1   ALAASPPROGLULYSARGLYSLEUILEGLN
2   GLNGLNLEUVALLEULEULEUHISALAHIS
3   LYSCYSGLNARGARGGLUGLNALAASNGLY
4   GLUVALARGALACYSSERLEUPROHISCYS
5   ARGTHRMETLYSASNVALLEUASNHISMET
6   THRHISCYSGLNALAGLYLYSALACYSGLN
7   VALALAHISCYSALASERSERARGGLNILE
8   ILESERHISTRPLYSASNCYSTHRARGHIS
9   ASPCYSPROVALCYSLEUPROLEULYSASN
10   ALASERASPLYSARG

Entity 3, Zinc ion, I - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-13C; U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_2: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-13C; U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; D2O 100%

Sample_3: hypoxia induced factor 1 alpha 2 mM; CREB-binding protein, [U-15N], 1 mM; ZINC (II) ION 3 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_4: hypoxia induced factor 1 alpha, [U-13C; U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

Sample_5: hypoxia induced factor 1 alpha, [U-13C; U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; D2O 100%

Sample_6: hypoxia induced factor 1 alpha, [U-15N], 1 mM; CREB-binding protein 2 mM; ZINC (II) ION 6 mM; D-TRIS 10 mM; D-DTT 10 mM; NaN3 0.02%; ZNSO4 0.5 mM; H2O 90%; D2O 10%

cond_1: ionic strength: 0.15 M; pH: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecond_1
1H-13C NOESYnot availablenot availablecond_1

Software:

No software information available

NMR spectrometers:

  • BRUKER DRX 800 MHz
  • BRUKER DRX 600 MHz

Related Database Links:

BMRB 5306 5987 6268
PDB
DBJ BAA78675 BAC28305 BAC28578 BAC36320 BAE01417 BAE06125 BAG65526
EMBL CAA64833 CAA70305 CAA70306 CAA70701 CAG29396
GenBank AAC50152 AAC51210 AAC52730 AAC53455 AAC53461 AAB28651 AAC08447 AAC51331 AAC51770 AAH72594
PRF 2114407A 1923401A
REF NP_001076251 NP_001116596 NP_001521 NP_034561 NP_077335 NP_001020603 NP_001073315 NP_004371 NP_596872 XP_001095225
SWISS-PROT O35800 Q0PGG7 Q16665 Q309Z6 Q61221 P45481 Q92793

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