BMRB Entry 4681

Title:
Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for apo-CRBPII
Deposition date:
2000-03-06
Original release date:
2000-03-20
Authors:
Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen
Citation:

Citation: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen. "The structure and dynamics of rat apo-cellular retinol-binding protein II in solution: comparison with the X-ray structure"  J. Mol. Biol. 286, 1179-1195 (1999).
PubMed: 10047490

Assembly members:

Assembly members:
Apo cellular retinol-binding protein II, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMON-CRBPII

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts548
1H chemical shifts1667
15N chemical shifts328

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apo CRBP II1

Entities:

Entity 1, Apo CRBP II 134 residues - Formula weight is not available

1   METTHRLYSASPGLNASNGLYTHRTRPGLU
2   METGLUSERASNGLUASNPHEGLUGLYTYR
3   METLYSALALEUASPILEASPPHEALATHR
4   ARGLYSILEALAVALARGLEUTHRGLNTHR
5   LYSILEILEVALGLNASPGLYASPASNPHE
6   LYSTHRLYSTHRASNSERTHRPHEARGASN
7   TYRASPLEUASPPHETHRVALGLYVALGLU
8   PHEASPGLUHISTHRLYSGLYLEUASPGLY
9   ARGASNVALLYSTHRLEUVALTHRTRPGLU
10   GLYASNTHRLEUVALCYSVALGLNLYSGLY
11   GLULYSGLUASNARGGLYTRPLYSGLNTRP
12   VALGLUGLYASPLYSLEUTYRLEUGLULEU
13   THRCYSGLYASPGLNVALCYSARGGLNVAL
14   PHELYSLYSLYS

Samples:

sample_1: Apo cellular retinol-binding protein II, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM

sample_2: Apo cellular retinol-binding protein II 1.5 mM

sample_3: Apo cellular retinol-binding protein II, [U-99% 15N], 1.5 mM

Ex-con_1: pH: 7.4; temperature: 298 K; ionic strength: 0.081 M

Ex-con_2: pH: 6.5; temperature: 298 K; ionic strength: 0.079 M

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian Unity 600 MHz

Related Database Links:

BMRB 4682
PDB
DBJ BAB22708
EMBL CAA52268
GB AAA42022 AAI25552 AAI25554 EDL20985 EDL77463
REF NP_033060 NP_036772 XP_006243669
SP P06768 Q08652
AlphaFold P06768 Q08652

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks