BMRB Entry 7251

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7251
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Title:
1H chemical shift assignments for wild type p53 tetramerization domain
Deposition date:
2006-08-08
Original release date:
2008-02-05
Authors:
Carbajo, Rodrigo; Mora, Puig; Sanchez del Pino, Manuel; Perez Paya, Enrique; Pineda-Lucena, Antonio
Citation:

Citation: Mora, Puig; Carbajo, Rodrigo; Pineda-Lucena, Antonio; Sanchez del Pino, Manuel; Perez Paya, Enrique. "Solvent-exposed residues located in the beta-sheet modulate the stability of the tetramerization domain of p53-A structural and combinatorial approach"  Proteins 71, 1670-1685 (2008).
PubMed: 18076077

Assembly members:

Assembly members:
p53TD, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53TD: EYFTLQIRGRERFEMFRELN EALELKDAQAG

Data sets:
Data typeCount
1H chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 21
3subunit 31
4subunit 41

Entities:

Entity 1, subunit 1 31 residues - Formula weight is not available

residues 326-356 p53 tetramerization domain

1   GLUTYRPHETHRLEUGLNILEARGGLYARG
2   GLUARGPHEGLUMETPHEARGGLULEUASN
3   GLUALALEUGLULEULYSASPALAGLNALA
4   GLY

Samples:

sample_1: p53TD wild type 0.5 mM; phosphate buffer 40 mM

conditions_1: pH: 7.2; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H,1H NOESYsample_1not availableconditions_1
1H,1H TOCSYsample_1not availableconditions_1

Software:

TOPSPIN v1.3, Bruker - Processing

SPARKY v3.110 - Assignment

CNS v1.1, Bruker - Structure calculations

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 4048 6521 6522
PDB
DBJ BAC16799 BAD96746 BAG35463 BAG59884 BAG60244
EMBL CAA25652 CAA26306 CAA34420 CAA38095 CAA42626
GB AAA17994 AAA59987 AAA59988 AAA59989 AAA61211
PRF 2006287A
REF NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590
SP P04637 P13481 P56423 P56424 P61260
AlphaFold P56424 P61260 P04637 P13481 P56423