BMRB Entry 6521
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6521
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Citation: Galea, Charles; Bowman, Prentice; Kriwacki, Richard. "Disruption of an intermonomer salt bridge in the p53 tetramerization domain
results in an increased propensity to form amyloid fibrils." Protein Sci. 14, 2993-3003 (2005).
PubMed: 16260757
Assembly members:
p53tet, polymer, 55 residues, 6265 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Vector: pET28a
Entity Sequences (FASTA):
p53tet: GSHMNNTSSSPQPKKKPLDG
EYFTLQIRGRERFEMFRELN
EALELKDAQAGKEPG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 98 |
| 15N chemical shifts | 98 |
| 13C chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit A | 1 |
| 2 | subunit B | 1 |
| 3 | subunit C | 1 |
| 4 | subunit D | 1 |
Entities:
Entity 1, subunit A 55 residues - 6265 Da.
Residues 10 to 60 correspond to the residues 310 to 360 of the p53 tetramerization domain.
| 1 | GLY | SER | HIS | MET | ASN | ASN | THR | SER | SER | SER | ||||
| 2 | PRO | GLN | PRO | LYS | LYS | LYS | PRO | LEU | ASP | GLY | ||||
| 3 | GLU | TYR | PHE | THR | LEU | GLN | ILE | ARG | GLY | ARG | ||||
| 4 | GLU | ARG | PHE | GLU | MET | PHE | ARG | GLU | LEU | ASN | ||||
| 5 | GLU | ALA | LEU | GLU | LEU | LYS | ASP | ALA | GLN | ALA | ||||
| 6 | GLY | LYS | GLU | PRO | GLY |
Samples:
sample_3: p53tet, [U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%
sample_1: p53tet, [U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%
sample_2: p53tet, [U-13C; U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%
sample_4: p53tet, [U-13C; U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%
conditions_1: pH: 6.0; temperature: 293.0 K
conditions_2: pH: 4.0; temperature: 293.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| 1H15N_NOESY | not available | not available | not available |
Software:
Felix, Accelrys -
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 4048 6522 7251 |
| PDB | |
| DBJ | BAC16799 BAD96746 BAG35463 BAG59884 BAG60244 |
| EMBL | CAA25652 CAA26306 CAA34420 CAA38095 CAA42626 |
| GB | AAA17994 AAA59987 AAA59988 AAA59989 AAA61211 |
| PRF | 2006287A |
| REF | NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590 |
| SP | P04637 P13481 P56423 P56424 P61260 |
| AlphaFold | P04637 P13481 P56423 P56424 P61260 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks