BMRB Entry 6521

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6521

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Title:
Characterization of an amyloid fibril intermediate
Deposition date:
2005-02-22
Original release date:
2007-01-29
Authors:
Galea, Charles; Bowman, Prentice; Kriwacki, Richard
Citation:

Citation: Galea, Charles; Bowman, Prentice; Kriwacki, Richard. "Disruption of an intermonomer salt bridge in the p53 tetramerization domain results in an increased propensity to form amyloid fibrils."  Protein Sci. 14, 2993-3003 (2005).
PubMed: 16260757

Assembly members:

Assembly members:
p53tet, polymer, 55 residues, 6265 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53tet: GSHMNNTSSSPQPKKKPLDG EYFTLQIRGRERFEMFRELN EALELKDAQAGKEPG

Data sets:
Data typeCount
1H chemical shifts98
15N chemical shifts98
13C chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1
4subunit D1

Entities:

Entity 1, subunit A 55 residues - 6265 Da.

Residues 10 to 60 correspond to the residues 310 to 360 of the p53 tetramerization domain.

1   GLYSERHISMETASNASNTHRSERSERSER
2   PROGLNPROLYSLYSLYSPROLEUASPGLY
3   GLUTYRPHETHRLEUGLNILEARGGLYARG
4   GLUARGPHEGLUMETPHEARGGLULEUASN
5   GLUALALEUGLULEULYSASPALAGLNALA
6   GLYLYSGLUPROGLY

Related Database Links:

BMRB 4048 6522 7251
PDB 1AIE 1C26 1OLG 1OLH 1PES 1PET 1SAE 1SAF 1SAK 1SAL 2J0Z 3SAK
DBJ BAC16799 BAD96746 BAG35463 BAG59884 BAG60244
EMBL CAA25652 CAA26306 CAA34420 CAA38095 CAA42626
GB AAA17994 AAA59987 AAA59988 AAA59989 AAA61211
PRF 2006287A
REF NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590
SP P04637 P13481 P56423 P56424 P61260
AlphaFold P04637 P13481 P56423 P56424 P61260

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks