BMRB Entry 4048

Title:
Solution Structure of the tetrameric minimum transforming domain of p53
Deposition date:
1997-07-28
Original release date:
1999-08-17
Authors:
Lee, Weontae; Harvey, Timothy; Yin, Ya; Yau, Patrick; Litchfield, David; Arrowsmith, Cheryl
Citation:

Citation: Lee, Weontae; Harvey, Timothy; Yin, Ya; Yau, Patrick; Litchfield, David; Arrowsmith, Cheryl. "Solution Structure of the tetrameric minimum transforming domain of p53"  Nat. Struct. Biol. 1, 877-890 (1994).

Assembly members:

Assembly members:
p53 nuclear phosphoprotein, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET19b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53 nuclear phosphoprotein: HMSTKRALPNNTSSSPQPKK KPLDGEYFTLQIRGRERFEM FRELNEALELKDAQAGKEPG GSRAHS

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts39
1H chemical shifts262

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1
4subunit D1

Entities:

Entity 1, subunit A 66 residues - Formula weight is not available

1   HISMETSERTHRLYSARGALALEUPROASN
2   ASNTHRSERSERSERPROGLNPROLYSLYS
3   LYSPROLEUASPGLYGLUTYRPHETHRLEU
4   GLNILEARGGLYARGGLUARGPHEGLUMET
5   PHEARGGLULEUASNGLUALALEUGLULEU
6   LYSASPALAGLNALAGLYLYSGLUPROGLY
7   GLYSERARGALAHISSER

Samples:

sample_one: p53 nuclear phosphoprotein 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%

sample_two: p53 nuclear phosphoprotein, [U-15N], 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%

sample_three: p53 nuclear phosphoprotein, [U-13C; U-15N], 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%

sample_conditions_one: pH: 7.0 na; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 600 600 MHz
  • Varian Unity +500 500 MHz

Related Database Links:

PDB
DBJ BAC16799 BAD96746 BAG35463 BAG59884 BAG60244
EMBL CAA25652 CAA26306 CAA34420 CAA38095 CAA42626
GB AAA17994 AAA59987 AAA59988 AAA59989 AAA61211
PRF 2006287A
REF NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590
SP P04637 P13481 P56423 P56424 P61260
AlphaFold P04637 P13481 P56423 P56424 P61260

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks