BMRB Entry 7101

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PDB ID: 2gjh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7101
MolProbity Validation Chart

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Title:
Mistranslation of a computationally designed protein yields an exceptionally stable homodimer: Implications for protein evolution and engineering.
Deposition date:
2006-05-05
Original release date:
2006-11-17
Authors:
Dantas, G.
Citation:

Citation: Dantas, G.; Watters, A.; Lunde, B.; Eletr, Z.; Isern, N.; Roseman, T.; Lipfert, J.; Doniach, S.; Tompa, M.; Kuhlman, B.; Stoddard, B.; Varani, G.; Baker, D.. "Mis-translation of a computationally designed protein yields an exceptionally stable homodimer: Implications for protein evolution and engineering."  J. Mol. Biol. 362, 1004-1024 (2006).
PubMed: 16949611

Assembly members:

Assembly members:
Designed protein TOP7, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Designed protein TOP7: MERVRISITARTKKEAEKFA AILIKVFAELGYNDINVTWD GDTVTVEGQLEGGSLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts59
1H chemical shifts390

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DESIGNED PROTEIN TOP7, chain 11
2DESIGNED PROTEIN TOP7, chain 21

Entities:

Entity 1, DESIGNED PROTEIN TOP7, chain 1 62 residues - Formula weight is not available

1   METGLUARGVALARGILESERILETHRALA
2   ARGTHRLYSLYSGLUALAGLULYSPHEALA
3   ALAILELEUILELYSVALPHEALAGLULEU
4   GLYTYRASNASPILEASNVALTHRTRPASP
5   GLYASPTHRVALTHRVALGLUGLYGLNLEU
6   GLUGLYGLYSERLEUGLUHISHISHISHIS
7   HISHIS

Related Database Links:

PDB 2GJH

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