BMRB Entry 6857

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6857

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Title:
Solution structure of an immunodominant epitope of myelin basic protein (MBP) - Conformational dependence on environment of an intrinsically unstructured protein
Deposition date:
2005-10-09
Original release date:
2006-04-20
Authors:
Fares, Christophe; Libich, David; Harauz, George
Citation:

Citation: Fares, Christophe; Libich, David; Harauz, George. "Solution NMR structure of an immunodominant epitope of myelin basic protein"  FEBS J. 273, 601-614 (2006).
PubMed: 16420483

Assembly members:

Assembly members:
FF2, polymer, 18 residues, 2141.4 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FF2: QDENPVVHFFKNIVTPRT

Data sets:
Data typeCount
13C chemical shifts84
1H chemical shifts391

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FF21

Entities:

Entity 1, FF2 18 residues - 2141.4 Da.

1   GLNASPGLUASNPROVALVALHISPHEPHE
2   LYSASNILEVALTHRPROARGTHR

Samples:

sample_1: FF2, no, 2 mM; KCl, no, 100 mM

sample_2: FF2 5 mM; TFE, [U-2H], 30%

sample_3: FF2 1 mM; DPC, [U-2H], 100 mM

conditions_1: pH: 6.5; temperature: 298 K

conditions_2: pH: 6.5; temperature: 300 K

conditions_3: pH: 6.5; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D-TOCSY (using DIPSI-2)not availablenot availablenot available
2D-NOESYnot availablenot availablenot available
1H-13C HSQC (natural abundance)not availablenot availablenot available

Software:

XWINNMR v3.5, Bruker - processing

SPARKY v3.111, UCSF NMR Lab - assignment

ARIA v2.0, Jens Linge, Michael Nilges - Structure calculation/refinement

CNS v2.0, Jens Linge, Michael Nilges - Structure calculation/refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15131 18520 19186 20062 6100
PDB
DBJ BAB23830 BAC37705 BAE28256 BAE87162 BAE87443
EMBL CAA10804 CAA10805 CAA10806 CAA10807 CAA35179
GB AAA37720 AAA39496 AAA39497 AAA39499 AAA39500
PRF 0411226A 1003243A 1102236A
REF NP_001001546 NP_001020252 NP_001020261 NP_001020263 NP_001020272
SP P02686 P02687 P02688 P04370 P06906
TPG DAA16031
AlphaFold P02686 P02687 P02688 P04370 P06906