BMRB Entry 15131

Title:
NMR assignment of an intrinsically disordered protein under physiological conditions: the 18.5 kDa isoform of murine myelin basic protein
Deposition date:
2007-02-13
Original release date:
2007-06-26
Authors:
Libich, David; Monette, Martine; Robertson, Valerie; Harauz, George
Citation:

Citation: Libich, David; Monette, Martine; Robertson, Valerie; Harauz, George. "NMR assignment of an intrinsically disordered protein under physiological conditions: the 18.5 kDa isoform of murine myelin basic protein"  Biomol. NMR Assignments 1, 61-63 (2007).
PubMed: 19636827

Assembly members:

Assembly members:
MBP, polymer, 176 residues, 19421.5 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_22b(+)

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts281
1H chemical shifts571

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBP1

Entities:

Entity 1, MBP 176 residues - 19421.5 Da.

sequence includes a LEHHHHHH affinity tag (residues 169-176)

1   ALASERGLNLYSARGPROSERGLNARGSER
2   LYSTYRLEUALATHRALASERTHRMETASP
3   HISALAARGHISGLYPHELEUPROARGHIS
4   ARGASPTHRGLYILELEUASPSERILEGLY
5   ARGPHEPHESERGLYASPARGGLYALAPRO
6   LYSARGGLYSERGLYLYSASPSERHISTHR
7   ARGTHRTHRHISTYRGLYSERLEUPROGLN
8   LYSSERGLNHISGLYARGTHRGLNASPGLU
9   ASNPROVALVALHISPHEPHELYSASNILE
10   VALTHRPROARGTHRPROPROPROSERGLN
11   GLYLYSGLYARGGLYLEUSERLEUSERARG
12   PHESERTRPGLYALAGLUGLYGLNARGPRO
13   GLYPHEGLYTYRGLYGLYARGALASERASP
14   TYRLYSSERALAHISLYSGLYPHELYSGLY
15   ALATYRASPALAGLNGLYTHRLEUSERLYS
16   ILEPHELYSLEUGLYGLYARGASPSERARG
17   SERGLYSERPROMETALAARGARGLEUGLU
18   HISHISHISHISHISHIS

Samples:

15N-MBP: MBP, [U-15N], 1.47 mM; potassium chloride 100 mM; D2O, [U-2H], 10%

15N_13C-MBP: MBP, [U-13C; U-15N], 2.0 mM; potassium chloride 100 mM; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 277 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N-MBPisotropicsample_conditions_1
2D 1H-15N HSQC15N_13C-MBPisotropicsample_conditions_1
3D CBCA(CO)NH15N_13C-MBPisotropicsample_conditions_1
3D HNCACB15N_13C-MBPisotropicsample_conditions_1
3D H(CCO)NH15N_13C-MBPisotropicsample_conditions_1
3D HBHA(CO)NH15N_13C-MBPisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

CARA v1.5.3, Rochus Keller - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GB AAA37720 AAA39496 AAA39501 EDL09385
REF NP_001020426 NP_001020463 NP_034907 XP_006526519 XP_013204950
SP P04370
AlphaFold P04370

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks