BMRB Entry 6100

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6100

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignents of the 18.5kDa isoform of murine myelin basic protein (MBP)

Deposition date:

2004-02-12

Original release date:

2007-10-19

Authors:

Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George

Citation:

Citation: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George. "Letter to the Editor: Backbone resonance assignments of the 18.5kDa isoform of murine myelin basic protein (MBP)" J. Biomol. NMR 29, 545-546 (2004).
PubMed: 15243191

Assembly members:

Assembly members:
myelin basic protein, polymer, 176 residues, 19421.5 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet22b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myelin basic protein: ASQKRPSQRSKYLATASTMD HARHGFLPRHRDTGILDSIG RFFSGDRGAPKRGSGKDSHT RTTHYGSLPQKSQHGRTQDE NPVVHFFKNIVTPRTPPPSQ GKGRGLSLSRFSWGAEGQRP GFGYGGRASDYKSAHKGFKG AYDAQGTLSKIFKLGGRDSR SGSPMARRLEHHHHHH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	436
15N chemical shifts	154
1H chemical shifts	516

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rmMBP	1

Entities:

Entity 1, rmMBP 176 residues - 19421.5 Da.

1

ALA

SER

GLN

LYS

ARG

PRO

SER

GLN

ARG

SER

2

LYS

TYR

LEU

ALA

THR

ALA

SER

THR

MET

ASP

3

HIS

ALA

ARG

HIS

GLY

PHE

LEU

PRO

ARG

HIS

4

ARG

ASP

THR

GLY

ILE

LEU

ASP

SER

ILE

GLY

5

ARG

PHE

SER

GLY

ASP

ARG

GLY

ALA

PRO

6

LYS

ARG

GLY

SER

GLY

LYS

ASP

SER

HIS

THR

7

ARG

THR

HIS

TYR

GLY

SER

LEU

PRO

GLN

8

LYS

SER

GLN

HIS

GLY

ARG

THR

GLN

ASP

GLU

9

ASN

PRO

VAL

HIS

PHE

LYS

ASN

ILE

10

VAL

THR

PRO

ARG

THR

PRO

SER

GLN

11

GLY

LYS

GLY

ARG

GLY

LEU

SER

LEU

SER

ARG

12

PHE

SER

TRP

GLY

ALA

GLU

GLY

GLN

ARG

PRO

13

GLY

PHE

GLY

TYR

GLY

ARG

ALA

SER

ASP

14

TYR

LYS

SER

ALA

HIS

LYS

GLY

PHE

LYS

GLY

15

ALA

TYR

ASP

ALA

GLN

GLY

THR

LEU

SER

LYS

16

ILE

PHE

LYS

LEU

GLY

ARG

ASP

SER

ARG

17

SER

GLY

SER

PRO

MET

ALA

ARG

LEU

GLU

18

HIS

Samples:

sample_1: myelin basic protein, [U-15N; U-13C], 2 mM; 2,2,2-trifluoroethanol-d2, [2H], 30%

sample_cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N-HSQC	sample_1	not available	sample_cond_1
CBCANH	sample_1	not available	sample_cond_1
CBCA(CO)NH	sample_1	not available	sample_cond_1
HCC(CO)NH	sample_1	not available	sample_cond_1
HNCO	sample_1	not available	sample_cond_1

Software:

No software information available

NMR spectrometers:

Bruker AV 600 MHz

BMRB	15131
GB	AAA37720 AAA39496 AAA39501 EDL09385
REF	NP_001020426 NP_001020463 NP_034907 XP_006526519
SP	P04370
AlphaFold	P04370